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厦门市售甲鱼蛋携带病原菌情况初步调查Primary Investigation of Pathogenic Bacteria from Sold Terrapin Eggs in Xiamen
吴玉娟, 孔繁德, 徐淑菲, 王仍瑞
甲鱼蛋, MALDI-TOF-MS, 病原菌Terrapin Egg, MALDI-TOF-MS, Pathogen
《Advances in Microbiology》, Vol.3 No.2, 2014-06-18
Objective: To explore the situation of pathogens of Xiamenlocal terrapin eggs. Methods: The pathogens were isolated with standard methods. The pure cultures isolated from terrapin eggs were further specified by MALDI-TOF-MS, serological test and PCR methods. Results: 100% of 7 batches of Xiamen local terrapin eggs were contaminated with pathogenic bacteria according to MALDI- TOF-MS. 76 strains of isolated pathogenic bacteria fell into 16 genera and 26 species. All of the terrapin eggs carried five or more pathogenic bacteria. Conclusion: The results suggest that high- contamination rate, broad genera, coexistence of multiple species and isolation of pathogenic bacterium are very common phenomena. It’s very necessary to enforce surveillance of pathogenic bacteria in terrapin eggs.