标题:
硒化壳寡糖和硒化羧甲基壳聚糖中硒含量的测定Determination of Selenium in Chitooligosaccharide-Selenium and Selenium Carboxymethyl-Chitosan
作者:
全文颖, 覃仙玲, 曹鹏, 马堃, 范圣第, 金黎明
关键字:
：硒化壳寡糖, 硒化羧甲基壳聚糖, 邻苯二胺分光光度法, 二氨基联苯胺分光光度法Chitooligosaccharide-Selenium; Selenium Carboxymethyl-Chitosan; O-Phenylenediamine Spectrophotometric; Diaminobenzidine Spectrophotometric
期刊名称:
《Bioprocess》, Vol.1 No.1, 2011-09-29
摘要:
分别以亚硒酸钠与壳寡糖、羧甲基壳聚糖为原料，合成硒化壳寡糖和硒化羧甲基壳聚糖。分别用邻苯二胺分光光度法和二氨基联苯胺分光光度法测定其中的硒含量，两种方法测定的结果较为一致，测得硒化壳寡糖中硒含量分别为9.02 mg/g、8.82 mg/g，测得硒化羧甲基壳聚糖中硒含量分别为20.50 mg/g、20.24 mg/g。
Sodium selenite and chitooligosaccharide, carboxymethyl-chitosan, respectively, were used to synthesize chitooligosaccharide-selenium and selenium carboxymethyl-chitosan in this experiment by chemical method. The contents of selenium were tested by o-phenylenediamine and diaminobenzidine spectrophotometric methods. The results showed the consistence of the two methods. The contents of selenium in chitooligosaccharide-selenium were 9.02 mg/g, 8.82 mg/g, respectively. The contents of selenium in selenium carboxymethyl-chitosan were 20.50 mg/g、20.24 mg/g, respectively.