标题:
PD98059对H2O2诱导的人乳腺癌细胞凋亡及相关蛋白的影响The Effect of the PD98059 on H2O2 Induced Apoptosis and Associated Proteins of Human Breast Cancer Cells
作者:
周钱梅, 苏式兵
关键字:
PD98059, MEK, MCF-7, 细胞凋亡PD98059; MEK; MCF-7; Apoptosis
期刊名称:
《Hans Journal of Biomedicine》, Vol.1 No.2, 2011-10-28
摘要:
目的：探讨细胞外信号调节激酶的上游激酶(MEK)抑制剂PD98059对H2O2诱导的人乳腺癌MCF-7细胞凋亡的影响及其作用机制。方法：H2O2诱导乳腺癌MCF-7细胞凋亡，PD98059干预其细胞凋亡。荧光染料吖啶橙(AO)观察细胞凋亡的形态学变化；流式细胞术法进行凋亡细胞计数；Western blot法检测p53，PCNA，caspase-3、caspase-9和Bcl-2的表达。结果：H2O2刺激细胞4 h、12 h，明显诱导了MCF-7细胞凋亡。PD98059将H2O2诱导的细胞凋亡率分别降低了约4％、14％，与H2O2刺激细胞12 h比较，具有显著的统计学差异(P Objective: to study the effect of MEK inhibitor of PD98059 on apoptosis induced by H2O2 and its mechanisms in human breast cancer MCF-7 cells. Methods: H2O2 inducing apoptosis in MCF-7 cells was set to observe apoptosis and its associated proteins treated in the present or absent of PD98059. The cellular morphology was investigated by AO staining. Apoptosis ratio was analyzed by flow cytometry. The expres-sions of p53, PCNA, caspase-3, caspase-9 and Bcl-2 were observed using Western blot analysis. Results: apoptosis was observed with cells treated H2O2 for 4 or 12 h while the degree of apoptosis was greatly de-creased by 4% or 14% in MCF-7 cells treated with H2O2 and PD98059 together. There was significantly dif-ference between with the treatment of H2O2 and PD98059 in the presence of H2O2 for 12 h (P