标题:
聚谷氨酸/聚吡咯复合纳米颗粒的制备及神经毒性研究Preparation of PGlu/PPy Composite Nanoparticles and Neurotoxicity Evaluation
作者:
曾静雯, 黄忠兵, 尹光福
关键字:
聚吡咯纳米颗粒, 神经毒性, 轴突生长, 聚谷氨酸, 十二烷基硫酸钠PPy Nanoparticle; Neurotoxicity; Neural Outgrowth; PGlu; SDS
期刊名称:
《Material Sciences》, Vol.3 No.3, 2013-05-27
摘要:
采用化学氧化合成法分别制备了聚谷氨酸以及聚谷氨酸/十二烷基硫酸钠掺杂的聚吡咯纳米颗粒。通过四探针法、傅里叶红外光谱、扫描电子显微镜、激光粒度分析和zeta电位分析等方法分析其结构和形貌。通过分别与L929以及PC12细胞共培养表征其细胞毒性和神经毒性。结果表明，掺杂剂会影响聚吡咯纳米颗粒的表观形貌和电学特性。PGlu掺杂的PPy纳米颗粒具有良好的细胞相容性，十二烷基硫酸钠的加入有利于提高PPy颗粒的导电性，但对细胞存活率和神经轴突的生长有轻微的不利影响。Polypyrrole (PPy) nanoparticles doped with poly(glutamic acid) (PGlu) or PGlu/sodium dodecyl sulfate (SDS) were prepared by oxidation polymerization. The PPy nanoparticles were characterized via standard four-probe setup, FT-IR, SEM, Laser Particle Size Analyzer (LPSA) and zeta CAD. In order to evaluate the cytotoxicity and neurotoxicity, the PPy particles were co-cultured with L929 and PC12, respectively. The results show that the dopants have an effect on the morphology and conductivity of PPy nanoparticles. PGlu doped PPy nanoparticles have good cell compatibility. The addition of SDS is beneficial to improve the conductivity of PPy nanoparticles, however it is slightly to the disadvantage of cell viability and neuron growth.