标题:
nrDNA-ITS区序列在植物系统与进化研究中的应用Application of nrDNA-ITS Sequences in Plant Phylogeny and Evolution
作者:
刘锡红, 张磊, 李刚, 覃瑞, 刘虹
关键字:
ITS序列, 系统发育, 裸子植物, 被子植物, 高级结构ITS Sequence; Phylogenetic Development; Angiosperm; Gymnosperm; Advanced Structure
期刊名称:
《Botanical Research》, Vol.3 No.1, 2014-01-26
摘要:
本文就核rDNA (nrDNA)的ITS区序列在植物系统与进化研究中的应用进行了讨论。nrDNA是植物系统发育研究中广泛应用的重要核基因片段。5.8S rDNA把nrDNA的内转录间隔区分为ITS1和ITS2两部分。被子植物中ITS1的长度为165~298 bp，ITS2的长度为177~266 bp；裸子植物中ITS片段较长，且其长度变化主要由ITS1的长度变异所致。由于ITS序列变异较快，能够提供较丰富的变异位点和信息位点，已成为被子植物较低分类阶元的系统发育和分类研究中的重要分子标记，为探讨多倍体复合体网状进化关系，异源多倍体的起源提供了重要的系统学信息。The application of nuclear rDNA ITS sequences in plant phylogeny and evolution analysis was discussed in this paper. Nuclear rDNA is an important nuclear gene fragment, which had been widely used in phylogenetic studies. The internal transcribed spacer of nuclear rDNA was divided into ITS1 and ITS2 by 5.8S rDNA. The length of ITS1 ranges from 165 bp to 298 bp in angiosperms, and the length of ITS2 ranges from 177 bp to 266 bp. ITS sequence is relatively longer in gymnosperms. It much depends on the length of ITS1. As mutate quickly, ITS sequences are capable of providing many variable sites and informative sites, which has become an important molecular marker in plant systematic development and classification researches within low-order angiosperm categories. AT the same time, it can prove important systematic information for exploring polyploidy reticulate evolution and allopolyploid origins.