哈尔滨地区甘蓝根肿病的病原鉴定

Pathogen Identification of Cabbage Clubroot in Harbin Area

摘要: 本试验对哈尔滨地区甘蓝(Brassica oleracea L.)发生的根部肿大、地上部不能形成叶球的新病害进行了鉴定，通过形态学观察，致病力测定，初步确定该病是由芸薹根肿菌(Plasmodiophora brassicae Woron.)侵染引起的甘蓝根肿病。随后利用根肿菌的核糖体DNA (rDNA)内转录间隔区(internal transcribed spacer, ITS)的保守性和变异性，用根肿菌ITS区段特异性引物对病原菌DNA进行PCR扩增，并将测序结果在GenBank中进行同源性比对分析，分子鉴定与形态学鉴定结果一致。

Abstract: The pathogen causing clubroot on cabbage (Brassica oleracea L.) in Harbin area was identified in this study. By morphological observation and pathogenicity testing, this paper preliminary suspects that this disease is caused by Plasmodiophora brassica Woron. Then by using the conservation and variability of the internal transcribed spacer (ITS) of Plasmodiophora ribosome DNA, the sequence was analyzed by comparing with that in GenBank. The molecular identification indicated the same results with morphology identification.