伊犁部分牛边缘无浆体与牛巴贝斯虫混合感染情况报告
The Report of Anaplasma marginale and Babesia bovis Mixed Infection in Cattle in Part of Area Yili
DOI: 10.12677/ACRPVM.2016.51001,
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作者:
孟 元, 吴 敏:浙江大学生科院,浙江 杭州;伊春阳, 明•乌尔娜, 张 杨, 巴音查汗:新疆农业大学动物医学学院,新疆 乌鲁木齐
关键词:
伊犁;边缘无浆体;巴贝斯虫;感染率
Yili; Anaplasma marginale; Babesia bovis; Infection Rate
摘要:
为探究新疆伊犁部分地区牛边缘无浆体与牛巴贝斯虫病的感染情况。本试验通过采集伊犁新源和特克斯县疑似牛全血,并根据16S rRNA基因保守序列,设计并合成引物,提取病原DNA,进行PCR检测。结果显示,新源县牛边缘无浆体的感染率为20%(40/200)而牛巴贝斯虫的感染率为40%(80/200),其混合感染率为30%(60/200);特克斯县牛边缘无浆体的感染率为30%(120/400)而牛巴贝斯虫的感染率为40%(160/400),混合感染率高达45%(180/400)。为我区牛边缘无浆体与牛巴贝斯虫病的防治提供数据基础。
Abstract:
To explore the situation of Anaplasma marginale and Babesia bovis mixed infection in part of area Yili, Xinjiang, this experiment collected suspected bovine blood in Xinyuan and Tekes county of Yili and designed and synthesized primer based on 16S rRNA gene conserved sequence, DNA extraction for PCR detection. Results showed that: Anaplasma marginale infection rate was 20%(40/200)and Babesia bovis infection rate was 40%(80/200); mixed infection rate was 30%(60/200)in cattle in Xinyuan county; Anaplasma marginale infection rate was 30%(120/400) and Babesia bovis infection rate was 40%(160/400), and mixed infection rate was as high as 45%(180/400)in Tekes county. These results provide the data base for Anaplasmosis and Babesiosis prevention in cattle Xnjiang.
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