HJAS  >> Vol. 4 No. 5 (October 2014)

    基于13项形态学指标的远东黑蜂形态变异分析
    Morphometric Analysis of Far East Blank Bee Based on 13 Indexes of Morphometry

  • 全文下载: PDF(594KB) HTML    PP.87-98   DOI: 10.12677/HJAS.2014.45014  
  • 下载量: 1,932  浏览量: 6,175   国家自然科学基金支持

作者:  

王进州,薛运波,张 发,李兴安:吉林省蜜蜂遗传育种重点实验室(吉林省养蜂科学研究所),吉林;
金水华:浙江平湖畜牧局蜜蜂育种场,平湖

关键词:
远东黑蜂形态变异分析尺度类形态学指标第四蜡镜Far East Blank Bee Morphometric Analysis Size Characters of Morphometry Proboscis Wax Mirror IV

摘要:

为了积累远东黑蜂的形态学数据,和以此数据为依据表征远东黑蜂,本研究首先通过显微图像分析方法分别测定了10群远东黑蜂样本和10群欧洲黑蜂样本各自的13项尺度类形态学指标;其次,通过F-分析比较了远东黑蜂样本测定值均数与欧洲黑蜂样本测定值均数的离散程度,通过t-检验比较了远东黑蜂样本测定值均数与欧洲黑蜂样本测定值均数的差异性。远东黑蜂样本的吻长度、第四蜡镜宽度、第四蜡镜面积测定值均数,都明显地小于欧洲黑蜂样本的相应测定值均数(P < 0.05);相反,它们之间的第三背板宽度、第四背板长度、第四背板宽度、第四蜡镜长度、前翅长度、前翅宽度、前翅面积、前翅肘脉指数、后腿跗节长度以及后腿跗节宽度测定值均数,都没有差异(P > 0.05)。这提示,相对于欧洲黑蜂,远东黑蜂具有明显的形态学体征:短的吻长度,短的第四蜡镜宽度和小的第四蜡镜面积。

A Microscopic image analysis was conducted to collect a set of morphometric data for characterizing morphological features of Far East Blank Bee. First of all, we measured 13 indexes of morphometry of 10 colonies of both Far East Blank Bee and Apis mellifera mellifera, respectively. Then we compared discreteness of means of measured values between them, and compared difference of the means between them. Compared to samples of colonies of Apis mellifera mellifera in the measurements of proboscis length, width and surface area of wax mirror IV, samples of colonies of Far East Blank Bee are showed with shorter proboscis, shorter wax mirror IV, and smaller surface area of wax mirror IV (P < 0.05, respectively). On the contrary, compared to samples of the former with the measurements of the others, samples of the latter are not showed with significant differ-ence of measurements, including width of tergum III, both length and width of tergum IV, length of wax mirror IV, length, width, surface area, cubital index of forewing, and both length and width of metabasitarsus hindleg (P > 0.05, respectively). The present analysis could distinguish the mor-phometric difference between the samples of colonies of both Far East Blank Bee and Apis mellifera mellifera.

文章引用:
王进州, 薛运波, 张发, 金水华, 李兴安. 基于13项形态学指标的远东黑蜂形态变异分析[J]. 农业科学, 2014, 4(5): 87-98. http://dx.doi.org/10.12677/HJAS.2014.45014

参考文献

[1] Crane, E. (1978) The Varroa mite. Bee World, 59, 164-167.
[2] Rinderera, T.E., de Guzmana, L.I., Delattea, G.T., Stelzera, J.A., Lancasterb, V.A., Kuznetsovc, V., Beamana, L., Wattsa, R. and Harrisa, J.W. (2001) Resistance to the parasitic mite Varroa destructor in honey bees from far-eastern Russia. Apidologie, 32, 381-394.
[3] Radloff, S.E., Hepburn1, C., Hepburn, H.R., Fuchs, S., Hadisoesilo, S., Tan, K., Engel, M.S. and Kuznetsov, V. (2010) Population structure and classification of Apis cerana. Apidologie, 41, 589-601.
[4] Vesala, L., Salminen, T. S., Laiho, A., Hoikkala, A. and Kankare, M. (2012) Cold tolerance and cold-induced modulation of gene expression in two Droso-phila virilis group species with different distributions. Insect Molecular Biology, 21, 107-118.
[5] Gregory, P.G., Evans, J.D., Rinderer, T. and de Guzman, L. (2005) Conditional immune-gene suppression of honeybees parasitized by Varroa mites. Journal of Insect Science, 5, 7.
[6] Schöning, C., Gisder, S., Geiselhardt, S., Kretschmann, I., Bi-enefeld, K., Hilker, M. and Genersch, E. (2012) Evidence for damage-dependent hygienic behaviour towards Varroa destructor-parasitised brood in the western honey bee, Apis mellifera. The Journal of Experimental Biology, 215, 264-271.
[7] Le Conte, Y. and Navajas, M. (2008) Climate change: Impact on honey bee populations and diseases. Revue Scientifique et Technique, 2, 485-497.
[8] Brandorf, A., Ivoilova, M., 李兴安, 薛运波 (2013) 俄罗斯蜜蜂保种群的遗传标识码: 基因组微卫星标记谱图. 中国蜂业, 07-09, 17-22.
[9] Bourgeois, L., Sheppard, W.S., Sylvester, H.A. and Rinderer, T.E. (2010) Genetic stock identification of Russian honey bees. Journal of Economic Entomology, 103, 917-924.
[10] Han, F., Wallberg, A. and Webster, M.T. (2012) From where did the Western honeybee (Apis mellifera) originate? Ecology and Evolution, 2, 1949-1957.
[11] Bouga, M., Alaux, C., Bienkowska, M., Büchler, R., Carreck, N. L., Cauia, E., Chlebo, R., Dahle, B., Dall’Olio, R., De la Rúa, P., Gregorc, A., Evgeniya, I., Kence, A., Kence, M., Kezic, N., Kiprijanovska, H., Kozmus, P., Kryger, P., Le Conte, Y., Lodesani, M., Murilhas, A.M., Siceanu, A., Soland, G., Uzunov, A. and Wilde, J. (2011) A review of methods for discrimination of honey bee populations as applied to European beekeeping. Journal of Apicultural Research, 50, 51-84.
[12] Alpatov, W.W. (1976) Rokoyava oshibka v opredelenii parody pchel [A fatal error in determining the race of a honeybee]. Priroda, 5, 72-73. (In Russian).
[13] 葛凤晨, 石巍, 王素芝, 刘先蜀, 张大隆, 罗岳雄, 梁勤, 颜志立, 薛运波 (2011) 中国蓄禽遗传资源志 (蜂蜜志).中国农业出版社, 北京.
[14] Dade, H.A. (2009) Anatomy and dissection of the honeybee. International Bee Research Association, London.
[15] Il’yasov, R.A., Petukhov, A.V., Poskryakov, A.V. and Nikolenko, A.G. (2007) Local honeybee (Apis mellifera mellifera L.) populations in the Urals. Genetika, 43, 855-858.
[16] Oleksa, A., Wilde, J., Tofilski, A. and Chybicki, I.J. (2013) Partial reproductive isolation between European subspecies of honey bees. Apidologie, 44, 611-619.
[17] Koeniger, N. and Gudrun, K. (2000) Reproductive isolation among species of the genus Apis. Apidologie, 31, 313-339.
[18] Kawamoto, T. (2003) Use of a new adhesive film for the preparation of multi-purpose fresh-frozen sections from hard tissues, whole-animals, insects and plants. Archives of Histology and Cytology, 66, 123-43.
[19] Rattanawanneea, A., Chanchaob, C. and Wongsiric, S. (2012) Geometric morphometric analysis of giant honeybee (Apis dorsata Fabricius, 1793) populations in Thailand. Journal of Asia-Pacific Entomology, 15, 611-618.
[20] Kandemir, I., Kence, M. and Kence, A. (2005) Morphometric and electrophoretic variation in different honeybee (Apis mellifera L.) Populations. Turkish Journal of Veterinary and Animal Sciences, 29, 885-890.
[21] De Anna, E.B. (2013) Insects as indicators of environmental stress (For the Degree of Doctor of Philosophy in Biological Sciences). College of Arts and Sciences, University of South Carolina, South Carolina.
[22] Benítez, H.A., Briones, R. and Jerez, V. (2010) Intra and Inter-population morphological variation of shape and size of the Chilean magnificent beetle, Ceroglossus chilensis in the Baker River Basin. Chilean Patagonia Journal of Insect Science, 11, 1-8.
[23] Mărghitas, L., Paniti-Teleky, O., Dezmirean, D., Mărgăoan, R., Bojan, C., Corona, C., Laslo, L. and Moise, A. (2008) Morphometric differences between honey bees (Apis mellifera carpatica) populations from transyluanian area. Lucrări stiinŃifice Zootehnie si Biotehnologii, 41, 309-315.
[24] Winston, M.L. (1987) The biology of the honey bee. Harvard University Press Cambridge, London.