AMB  >> Vol. 4 No. 1 (March 2015)

    Preliminary Discussion on Problems of Rhizobium Inoculants

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李剑峰,张淑卿,韩 晗,张丽丽:贵州师范学院,贵州省生物资源开发利用特色重点实验室,喀斯特生境土壤与环境生物修复研究所,贵州 贵阳;
杜建雄:贵州财经大学,贵州 贵阳

根瘤菌商用菌剂污染产品质量Rhizobium Commercial Inoculants Contamination Product Quality



Rhizobium is found excellent in promoting growth and yield of legume crops, which decreases the cost of agricultural production and the side effect of chemical fertilizer application. Currently, Rhizobium inoculants have not been widely used in agriculture. Currently, the practical applications of Rhizobium are restricted by quality factors of some inoculants. In this paper, we discussed briefly the factors such as low strain breeding efficiency of Rhizobium, serious contamination and short shelf life of inoculants, to provide some reference to the further research and application of commercial Rhizobium inoculants.

李剑峰, 张淑卿, 杜建雄, 韩晗, 张丽丽. 根瘤菌剂现存问题初探[J]. 微生物前沿, 2015, 4(1): 20-25.


[1] 干大木, 赵小琴, 周义, 等 (2009) BGB 草莓专用微生物菌剂在草莓生产上的应用试验. 南方农业, 4, 8-9.
[2] 师尚礼 (2005) 紫花苜蓿根瘤菌研究进展. 甘肃农业大学学报, 2, 262-267.
[3] Rossi, M.J., Agenor, F.J. and Oli-veira, V.L. (2007) Inoculant production of ectomycorrhizal fungi by solid and submerged fermentations. Food Tech-nology and Biotechnology, 45, 277-286.
[4] Kenney, D.S. (1997) Commercialization of biological control products in the chemical pesticide world. In: Ogoshi, A., Kobayashi, K., Homma, Y., et al., Eds., Plant Growth—Promoting Rhizobacteria—Present Status and Future Prospects, Faculty of Agriculture, Sapporo University, Sapporo, 120-127.
[5] Schwartz, M.W., Hoeksema, J.D., Gehring, C.A., et al. (2006) The promise and the potential conse-quences of the global transport of mycorrhizal fungal inoculum. Ecology Letters, 9, 501-515.
[6] Bashan, Y. (1998) Inoculants of plant growth-promoting bacteria for use in agriculture. Biotechnology Advances, 16, 729-770.
[7] Olsen, P.E., Rice, W.A. and Collins, M.M. (1994) Biological contaminants in North American legume inoculants. Soil Biology and Biochemistry, 27, 699-701.
[8] Gomez, M., Silva, N., Hartmann, A., et al. (1997) Evaluation of commercial soybean inoculants from Argentina. World Journal of Microbiology and Biotechnology, 13, 167-173.
[9] Lupwayi, N.Z., Clayton, G.W. and Rice, W.A. (2006) Rhizobial inoculants for legume crops. Journal of Crop Improvement, 15, 289-321.
[10] Catroux, G., Hartmann, A. and Revellin, C. (2001) Trends in rhizobial inoculant production and use. Plant and Soil, 230, 21-30.
[11] Albareda, M., Rodríguez-Navarro, D.N., Camacho, M. and Temprano, F.J. (2008) Alternatives to peat as a carrier for rhizobia inoculants: Solid and liquid formulations. Soil Biology and Biochemistry, 40, 2771-2779.
[12] 中华人民共和国农业部 (2001) 根瘤菌肥料标准NY410-2000. 中国标准出版社, 北京, 1-9.
[13] Paau, A.S. (1988) Formulations useful in applying beneficial microorganisms to seeds. Trends in Biotech-nology, 6, 276-279.
[14] Jung, G., Mugnier, J., Diem, H.G. and Dommergues, Y.R. (1982) Polymer-entrapped rhizo-bium as an inoculant for legumes. Plant and Soil, 65, 219-231.
[15] Marufu, L., Karanja, N. and Ryder, M. (1995) Legume inoculant production and use in East and Southern Africa. Soil Biology and Biochemistry, 27, 735-738 .
[16] Thompson, J.A. (1991) Australian quality control and standards. In: Thompson, J.A., Ed., Report of the Expert Consultation on Legume Inoculant Production and Quality Control, Food and Agriculture Organization of the United Nations, Rome, 107-111.
[17] Lupwayi, N.Z., Olsen, P.E., Sande, E.S., Keyser, H.H., Collins, M.M., Singleton, P.W. and Rice, W.A. (2000) Inoculant quality and its evaluation. Field Crops Research, 65, 259-270.
[18] Smith, R.S. (1992) Legume inoculant formulation and application. Canadian Journal of Microbiology, 38, 485-492.
[19] Rossi, M.J., Agenor, F.J. and Oliveira, V.L. (2007) Inoculant production of ectomycorrhizal fungi by solid and submerged fermentations. Food Technology & Biotechnology, 45, 277-286.
[20] 常玮, 王炜, 屈新兰 (2004) 苜蓿根瘤菌菌剂的研究. 新疆农业科学, 2, 102-104.
[21] 张会春 (1999) 细菌肥料的研究与生产现状. 农业与技术, 19, 38-40.
[22] 中华人民共和国农业部 (2009) 根瘤菌生产菌株质量评价技术规范. NY/T 1735-2009, 北京.
[23] Kosslak, R.M. and Bohlool, B.B. (1985) Influence of environmental factors on interstrain competition in Rhizobium japonicum. Applied and Environmental Microbiology, 49, 1128-1133.
[24] Sessitsch, A., Jjemba, P.K., Hardarson, G., Akkermans, A.D.L. and Wilson, K.J. (1998) Measurement of the competitive index of Rhizobium tropici strain CIAT899 derivatives marked with the gusA gene. Soil Biology and Biochemistry, 29, 1099-1110.
[25] Kumar, R. and Chandra, R. (2008) Influence of PGPR and PSB on Rhizobium leguminosarum Bv. viciae strain competition and symbiotic performance in lentil. World Journal of Agricultural Sciences, 4, 297-301.
[26] 李剑峰, 张淑卿, 师尚礼 (2009) 微波诱变选育高产生长素及耐药性根瘤菌株研究. 核农学报, 6, 981-985.
[27] 李剑峰 (2011) 解磷根瘤菌诱变选育及抗污染菌剂制备关键技术研究. 甘肃农业大学, 兰州, 87-112.
[28] Chaiharn, M. and Lumyong, S. (2011) Screening and optimization of indole-3-acetic acid production and phosphate solubilization from rhizobacteria aimed at improving plant growth. Current Microbiology, 62, 173-181.
[29] Fukuhara, H., Minakawa, Y., Akao, S. and Minamisawa, K. (1994) The involvement of indole-3-acetic acid produced by Bradyrhizobium elkanii in nodule formation. Plant and Cell Physiology, 35, 1261-1265.
[30] Remans, R., Ramaekers, L., Schelkens, S., Hernandez, G., Garcia, A., Reyes, J.L., et al. (2008) Effect of Rhizobium- Azospirillum coinoculation on nitrogen fixation and yield of two contrasting Phaseolus vulgaris L. genotypes cultivated across different environments in Cuba. Plant and Soil, 312, 25-37.
[31] Zaied, K.A., Kosba, Z.A., Nassef, M.A. and El-saied, A.I. (2009) Induction of Rhizobium inoculants harboring salicylic acid gene. Australian Journal of Basic and Applied Sciences, 3, 1386-1411.
[32] 师尚礼, 曹致中, 刘建荣 (2007) 苜蓿根瘤菌溶磷和分泌植物生长素能力研究. 草业学报, 1, 105-111.
[33] 祁娟 (2006) 苜蓿种子根瘤菌筛选及其促生能力研究. 甘肃农业大学, 兰州.
[34] Halder, A.K. and Chakrabartty, P.K. (1993) Solubilization of inorganic phosphate by Rhizobium. Folia Microbiologica, 38, 325-330.
[35] 李剑峰, 张淑卿, 师尚礼, 霍平慧 (2009) 微波诱变选育耐药高效溶磷苜蓿根瘤菌. 原子能科学技术, 12, 1071-