海尔福治疗对铝造模阿尔茨海默病小鼠大脑神经损害后再生效果的研究

The Curative Effect of HaierFu Liquid on Alzheimer’s Disease by Aluminum Lead Poisoned Mice

摘要: 目的：探讨铝造模阿尔茨海默病小鼠大脑神经损害后，海尔福口服液对其再生的治疗效果。方法：选用昆明种小白鼠48只，随机分为4组：正常组、模型组、治疗1组、治疗2组，每组12只。后3组用氯化铝和硝酸铝喂养3个月，复制铝染毒动物模型。染毒2个月后，治疗1组、治疗2组分别用大、小不同剂量的中药海尔福口服液灌胃治疗1个月，正常组和模型组用等体积的蒸馏水灌胃对照。测定实验前和试验后的血红蛋白(Hb)。实验结束后，分别测小鼠血清和脑匀浆的各项生化指标。并取部分大脑用10%甲醛溶液固定后，作病理检查，对比分析。结果：模型组脑匀浆乙酰胆碱酯酶(TchE)活性明显低于其他三组，(P < 0.01或P < 0.05)，而模型组脑匀浆丙二醛(MDA)却显著高于治疗组(P < 0.01或P < 0.05)，氧自由基清除率正常组最高，模型组最低。大脑病理检查结果显示，铝中毒模型组的光镜结构，大脑皮质分层不清楚，外颗粒层颗粒和内颗粒细胞数量明显减少，胞质的尼氏体也减数量少且体积变小。有较多的神经元萎缩、变性；治疗组的病理改变得到很好改善，颗粒细胞数量增多，神经元胞质尼氏体也数量增多体积增大，其中治疗2组病理改变得到改善较明显。结论：铝致阿尔茨海默病小鼠脑组织TchE活性和氧自由基清除率明显下降，大脑神经元受损害，海尔福口服液治疗后明显好转。

Abstract: Objective: To explore the curative effect of HaierFu liquid on Alzheimer’s disease by aluminum lead poisoned mice. Methods: 48 Kunming white mice were divided into 4 groups: normal group, model group, treated group 1 and treated group 2. There were 12 rats in each group. Mice of the later three groups were fed with AlCl3 and Al(NO3)3 for three months to make aluminum lead poisoned mice model. After being poisoned two months, mice of treated group 1 and treated group 2 were poured with different dosages of Chinese medicine—HaierFu liquid for one month, and mice of normal group and model group were poured with equal pure water at the same time. Blood Hemoglobin (Hb) was determined before and after experiment, and biochemical indices of serum and brain tissue were determined at the end of experiment. Result: The vitality of TchE in brain tissue of model group was obviously lower than that of other groups (P < 0.01 or P < 0.05). However, the content of MDA in brain tissue of model group was obviously higher than that of treated group (P < 0.01 or P < 0.05), and for the clearance rate of oxygen-free radicals, normal group was the highest while model group was the lowest. There was no obvious difference of Hb and Tc in serum, and ALT in serum among each group. Conclusion: The vitality of TchE in brain tissue and the clearance rate of oxygen-free radicals in Alzheimer’s disease by aluminum lead poisoned mice were obviously decreased; HaierFu liquid have an obvious curative effect on Alzheimer’s disease by aluminum lead poisoned mice.