学术期刊
切换导航
首 页
文 章
期 刊
投 稿
预 印
会 议
书 籍
新 闻
合 作
我 们
按学科分类
Journals by Subject
按期刊分类
Journals by Title
核心OA期刊
Core OA Journal
数学与物理
Math & Physics
化学与材料
Chemistry & Materials
生命科学
Life Sciences
医药卫生
Medicine & Health
信息通讯
Information & Communication
工程技术
Engineering & Technology
地球与环境
Earth & Environment
经济与管理
Economics & Management
人文社科
Humanities & Social Sciences
合作期刊
Cooperation Journals
首页
生命科学
植物学研究
Vol. 5 No. 3 (May 2016)
期刊菜单
最新文章
历史文章
检索
领域
编委
投稿须知
文章处理费
最新文章
历史文章
检索
领域
编委
投稿须知
文章处理费
外壁破裂对油菜小孢子胚胎发生影响的显微观察
Ultrasturctural Studies on Broken Exine Roles for Microspore Embryogenesis in Brassica napus L.cv. Topas
DOI:
10.12677/BR.2016.53014
,
PDF
,
HTML
,
XML
,
被引量
下载: 1,814
浏览: 5,962
国家自然科学基金支持
作者:
涂金华
,
万佳馨
:湖北大学生命科学学院,湖北 武汉;
何宇清
:武汉大学生命科学学院,湖北 武汉;
汤行春
*
:湖北大学生命科学学院,湖北 武汉;武汉大学生命科学学院,湖北 武汉
关键词:
油菜
;
裂外壁小孢子
;
胚胎发生
;
超微结构
;
Brassica napus L.cv Topas
;
Exine-Dehisced Microspore
;
Embryogenesis
;
Ultrasturcture
摘要:
前期研究表明,油菜裂外壁小孢子也能胚胎发生。本研究以油菜裂外壁小孢子为材料,利用超微结构显微观察了破裂的外壁对胚胎发生的影响。结果表明,裂外壁小孢子可表现出与合子相似的某些结构特征;与合子进行不均等分裂而形成形态、结构迥异的顶基细胞类似,裂外壁小孢子也可以进行不均等分裂,形成大小和结构不同的两个子细胞。这些结果暗示破裂的外壁在小孢子极性生长和启动胚胎发生过程中重要作用。
Abstract:
In prior studies, the exine-dehisced microspore could also embryogenesis. Early events in micro-spore embryogenesis, such as polarity establishment, cell wall development, and the first division plane decision were investigated to reveal the mechanism controlling the process. In this paper, ra-peseed exine-dehisced microspores were used to reveal the roles of exine for microspore embryo-genesis by ultrastructural observation. The results show that some ultrastructural characteristic of the microspore is comparable to that of zygote, and that the dividing behavior of some exine-de- hisced microspore is similar to that of zygotes which could divide asymmetrically, resulting in two different daughter cells. This observation suggested that broken exine may play an important role in the polarity establishment during microspore embryogenesis.
文章引用:
涂金华, 万佳馨, 何宇清, 汤行春. 外壁破裂对油菜小孢子胚胎发生影响的显微观察[J]. 植物学研究, 2016, 5(3): 100-105.
http://dx.doi.org/10.12677/BR.2016.53014
参考文献
[
1
]
田辉, 孙蒙祥. 从油菜裂外壁小孢子培育出胚状体与小植株. 作物学报, 2003, 29(01): 49-53.
[
2
]
Tang, X., Liu, Y., He, Y., Ma, L., Sun, M. X. (2013) Exine Dehiscing Induces Rape Microspore Polarity, which Results in Different Daughter Cell Fate and Fixes the Apical-Basal Axis of the Embryo. Journal of Experimental Botany, 64, 215-228.
http://dx.doi.org/10.1093/jxb/ers327
[
3
]
He, Y.C., He, Y.Q., Qu, L.H., Sun, M.X. and Yang, H.Y. (2007) To-bacco Zygotic Embryogenesis in Vitro: The Original Cell Wall of the Zygote Is Essential for Maintenance of Cell Polarity, the Apical-Basal Axis and Typical Suspensor Formation. Plant Journal, 49, 515-527
http://dx.doi.org/10.1111/j.1365-313X.2006.02970.x
[
4
]
Hause, B., van Veenendaal, W.L.H., Hause, G. and van Lammeren, A.A.M. (1994) Expression of Polarity during Early Development of Microspore-Derived and Zygote Embryos of Brassica napus L.cv. Topas. Botanica Acta, 6, 369-472.
[
5
]
Lichter, R. (1982) Induction of haploid plants from isolated pollen of Brassica napus. Zeitschrift für Pflanzenphysiologie, 105, 427-434.
http://dx.doi.org/10.1016/S0044-328X(82)80040-8
[
6
]
Knox, E.B. (1984) The Pollen Grain. In: Johri, B.M., Ed., Embryology of Angiosperms, Springer-Verlag, Berlin, Heidelberg, New York and Tokyo, 197-271.
http://dx.doi.org/10.1007/978-3-642-69302-1_5
[
7
]
余凤群, 傅丽霞, 刘后利, 翟波, 金晓萍. 油菜小孢子胚胎发生的超微结构和胚状体形态[J]. 西北植物学报, 1997(17): 181-186.
[
8
]
Zaki, M.A.M. and Dickinson, H.G. (1990) Structural Changes During the First Divisions of Embryos Resulting from Anther and Free Microspore Culture in Brassica napus. Protoplasma, 156, 149-162.
http://dx.doi.org/10.1007/BF01560653
[
9
]
Thorpe, T.A. and Murashige, T. (1970) Some Histochemical Changes Underlying Shoot Initiation in Tobacco Callus Cultures. Canadian Journal of Botany, 48, 277-285.
http://dx.doi.org/10.1139/b70-042
[
10
]
崔凯荣, 等. 植物体细胞胚胎发生研究的某些现状[J]. 植物学通报, 1993, 10(3): 14-20.
[
11
]
王亚馥, 崔凯荣, 汪丽虹. 小麦体细胞胚发生的超微结构研究[J]. 植物学报, 1994(36): 418-422.
[
12
]
刘春明. 陆地棉体细胞胚发生及其细胞组织学研究[J]. 植物学报, 1991(33): 378-384.
[
13
]
Huang, B. (1982) Development Studies of Anther Cultures of Hordeum, Triticum and Paeonia. Ph.D. Thesis, University of East Anglia, Norwich.
[
14
]
Indrianto, A., Barinova, I., Touraev, A. and Heberle-Bors, E. (2001) Tracking Individual Wheat Microspores in Vitro: Identification of Embryogenic Microspores and Body Axis Formation in the Embryo. Planta, 212, 163-174.
http://dx.doi.org/10.1007/s004250000375
[
15
]
Jane, W.N. (1999) Ultrastructure of Embryo Development in Arundo formosana Hack (Poaceae). International Journal of plant Sciences, 160, 46-63.
http://dx.doi.org/10.1086/314101
[
16
]
Krishnan, H.B. and White, J.A. (1997) Protein Body Formation and Immunocytochemical Localization of Globulins and Glutelins in Developing Rice (Qryza sativa) Embryos. Crop Science, 37, 932-939.
http://dx.doi.org/10.2135/cropsci1997.0011183X003700030038x
投稿
为你推荐
友情链接
科研出版社
开放图书馆