HJCET  >> Vol. 6 No. 6 (November 2016)

    一株产纤维素酶甲醇耐受细菌的分离与鉴定
    Separation and Identification of a Bacterum Which Can Produce Cellulase and Tolerate Methanol

  • 全文下载: PDF(1254KB) HTML   XML   PP.163-168   DOI: 10.12677/HJCET.2016.66023  
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作者:  

嵇朝球:江苏石油勘探局洪泽农场,江苏 淮安;
祁鹏飞,裴 坤,马中良:上海大学生命科学学院,上海

关键词:
巨大芽孢杆菌纤维素酶16S rDNA B. megaterium Cellulose 16S rDNA Resistance Methanol

摘要:

当今时代,生物能源已经成为一种绿色经济的能源。为了更有效地利用纤维素,在这项研究中,自江苏盐城工业园区采集的土样中分离到一株既可耐受甲醇,又可产生高活性纤维素酶的细菌。通过16S rDNA序列分析,构建系统发育树,该菌株与巨大芽孢杆菌(B. megaterium)相似性为99.7%。甲醇耐受实验表明,菌株具有较高的甲醇耐受,甚至能在11.85%甲醇的M9培养基中生长。

Bioenergy is a green and economic energy in modern times. To have Celluose used more efficiently, in this study, from soil samples in Yancheng Industrial Park of Jiangsu, we isolated a bacterium that cannot only grow in the presence of methanol, but also produce higher activity cellulase Provice. By sequencing analysis of 16S rDNA, the phylogenetic tree was constructed, and the similarity between the strain and B. megaterium was 99.7%. The testing of tolerance in methanol showed that the strains had higher tolerance of methanol and could even grow in the M9 medium with 11.85% methanol.

文章引用:
嵇朝球, 祁鹏飞, 裴坤, 马中良. 一株产纤维素酶甲醇耐受细菌的分离与鉴定[J]. 化学工程与技术, 2016, 6(6): 163-168. http://dx.doi.org/10.12677/HJCET.2016.66023

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