MD  >> Vol. 6 No. 4 (December 2016)

    温栀子对溃疡性结肠炎治疗作用的研究
    Research on the Therapeutic Effects of Wen Gardenia on Ulcerative Colitis

  • 全文下载: PDF(1828KB) HTML   XML   PP.102-107   DOI: 10.12677/MD.2016.64018  
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作者:  

赵佳,钱益宇,沈施涟,张艳芬,马瑶,苏沉思,潘亮亮:温州医科大学仁济学院,浙江 温州

关键词:
溃疡性结肠炎温栀子超微结构Ulcerative Colitis Wen Gardenia Ultra Microstructure

摘要:

目的:探讨温栀子对溃疡性结肠炎的治疗效果及合适的治疗剂量。方法:48只SD大鼠采用DNCB/乙酸复合法建立溃疡性结肠炎模型,确定造模成功后,随机分成低、中、高剂量温栀子组、SASP阳性对照组及生理盐水对照组,分别予以相应治疗。从形态学、免疫学和病理学角度探究温栀子对溃疡性结肠炎的治疗作用。结果:经治疗,低、中、高剂量温栀子组大鼠症状、体征较生理盐水组均有好转,其中高剂量温栀子组溃疡性结肠炎改善最为明显,扫描电镜下显示结肠细胞表面平滑;病理切片显示温栀子治疗组黏膜结构均有所恢复;透射电镜下可见细胞结构恢复良好。其大体形态学评分、病理组织学等指标均较模型组有显著差异(P < 0.05)。结论:温栀子是治疗溃疡性结肠炎的有效药物。

Objective: To explore the therapeutic effect of Wen Gardenia Ellis dosage on ulcerative colitis and appropriate treatment dose. Method: 48 SD rats ulcerative colitis model were inoculated using DNCB/acetic acid method. After determining the successful models, they were randomly divided into low, medium and high dose dosage Wen Gardenia group, SASP positive control group and saline control group which were given their corresponding treatments. The morphology, immunology and pathology findings were used to explore the dosage of Wen Gardenia Ellis on ulcerative colitis. Re-sults: After the treatment, the group of rats treated with Wen gardenia had symptoms and signs which were improved when compared to the saline group, and the high dose group had the most significant improvement in the treatment of ulcerative colitis; under scanning electron microscope: colonic cell surface was smooth; pathology showed that the mucosal structure of rats that had the Wen Gardenia treatment was restored; cell structure was observed under transmission electron microscope and exhibited good recovery. The general morphology, histopathological score and other indicators were compared with the model group and the results showed that they were significantly different (P < 0.05). Conclusion: The dose of Wen Gardenia is an effective drug in the treatment of ulcerative colitis.

文章引用:
赵佳, 钱益宇, 沈施涟, 张艳芬, 马瑶, 苏沉思, 潘亮亮. 温栀子对溃疡性结肠炎治疗作用的研究[J]. 医学诊断, 2016, 6(4): 102-107. http://dx.doi.org/10.12677/MD.2016.64018

参考文献

[1] Wltilks, S. (1859) The Morid Appearance of the Intestine of Miss Banks. Medical Times and Gazette, 2, 264-269.
[2] Kornbluth, A. and Sachar, D.B. (1997) Ulcerative Colitis Practice Guidelines in Adults. American College of Gastroenterology Gastroenterology, Practice Parameters Committee. American Journal of Gastroenterology, 92, 204-11.
[3] 郭振军. 大黄多糖治疗溃疡性结肠炎的机制研究[D]: [博士学位论文]. 西安: 第四军医大学, 2013: 1-2.
[4] 任治军, 张立明, 何开泽. 栀子主要成分的提取工艺及药理研究进展[J]. 中国科学院成都生物研究所, 2005, 17(6): 834-835.
[5] 刘慧峰, 肖兵华, 李卉, 等. 高效液相色普法测定栀子I号方中华栀子苷的含量[J]. 江西中医学院学报, 2005, 17(1): 53-54.
[6] 江学良, 权启镇, 王东, 等. 复合法建立大鼠溃疡性结肠炎模型[J]. 青海医学院学报, 1999, 20(4): 1-3.
[7] 张涛, 谢建群, 等. 大鼠溃疡性结肠炎模型的实验研究[J]. 中国中西医结合消化杂志, 2006, 14(4): 240-242.
[8] 杜群, 李红, 王建华, 王汝俊. 溃结灵对溃疡性结肠炎大鼠治疗作用的病理学观察[J]. 中药新药与临床药理, 2007, 18(3): 173-175.
[9] 李建鹏, 潘亮亮,叶梁. 温栀子治疗溃疡性结肠炎的实验研究[J]. 浙江中医杂志, 2015, 50(5): 322-323.
[10] Lehmann, F.S. (2003) Pathophysiology of Inflammatory Bowel Disease. Ther Umsch, 60, 127-132.
https://doi.org/10.1024/0040-5930.60.3.127
[11] 欧阳钦. 炎症性肠病的病因和发病机理[J]. 临床内科杂志, 1998, 15(2): 59-60.
[12] Li, Y., Kamo, S., Metori, K., et al. (2000) The Promoting Effect of Eucommiol from Eucomiae Cortex on Collagen Synthesis. Biological and Pharmaceutical Bulletin, 23, 54-59.
https://doi.org/10.1248/bpb.23.54
[13] Playford, R.J. (1997) Trefoil Pep Tides: What Are They and What Do They Do? Journal of the Royal College of Physicians of London Royal College of Physicians of London, 31, 37-41.