Abstract:
A HPLC method was established to analyze Pyrazosulfuron-ethyl and Mefenacet simultaneously in WP formulation, using high performance liquid chromatography, with methanol and water as mobile phase, and ZORBAX SB-C18 5 µm column and ultraviolet detector at a wavelength of 250 nm. The linear correlation coefficients of 0.9995 and 0.9998 were obtained for Pyrazosulfuron-ethyl and Mefenacet, respectively.The standard deviations of 0.063% and 0.089% were obtained with the coefficient of variation of 1.4% and 0.13%. The average recoveries were 100.3%, 100.0%.