OJFR  >> Vol. 4 No. 3 (September 2017)

    氨氮胁迫对泥蚶免疫酶活性的影响
    Effects of Ammonia-N Stress on the Activities of Immune Enzymes of Tegillarca granosa

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作者:  

王程昊,钱琦凡,张耀尹,吴茜丹,金建钰:温州大学教师教育学院,浙江 温州

关键词:
泥蚶氨氮胁迫过氧化氢酶酸性磷酸酶碱性磷酸酶Tegillarca granosa Ammonia-N Stress Catalase Acid Phosphatase Alkaline Phosphatase

摘要:

本实验以泥蚶为研究对象,研究了不同浓度下的氨氮胁迫对三种免疫酶活性的影响。实验结果发现:1) 氨氮浓度对泥蚶过氧化氢酶(CAT)活力的影响不显著(F6,14 = 2.6074, P = 0.06545 > 0.05),随着氨氮浓度的增加,总体上CAT活性表现出“诱导–抑制”的趋势。2) 氨氮浓度极显著影响泥蚶酸性磷酸酶(ACP)的活力(F6,14 = 13.693, P = 0.00004 < 0.01),随着氨氮浓度的增加,总体上ACP活性表现出“抑制–诱导”的趋势。3) 氨氮浓度极显著影响泥蚶碱性磷酸酶(AKP)的活力(F6,14 = 13.696, P = 0.00004 < 0.01),随着氨氮浓度的增加,总体上AKP活性表现出“诱导–抑制”的趋势。结果表明,氨氮胁迫影响泥蚶这三种免疫酶的活性。

In this paper, the effects of ammonia-N stress on the activities of immune enzyme of Tegillarca granosa were studied at different conditions with ecological method. The results were as follows: 1) Ammonia-N concentration was found to have no significant effect on the activity of catalase (CAT) of Tegillarca granosa (F6, 14 = 2.6074, P = 0.06545 > 0.05). As the concentration of ammonia-N increased, CAT generally demonstrated an “induction-suppression” activity. 2) Ammonia-N concentration had a highly significant impact on the activity of acid phosphatase (ACP) of Tegillarca granosa (F6, 14 = 13.693, P = 0.00004 < 0.01). As the concentration of ammonia-N increased, ACP generally demonstrated a “suppression-induction” activity. 3) Ammonia-N concentration had a highly significant impact on the activity of alkaline phosphatase (AKP) of Tegillarca granosa (F6, 14 = 13.696, P = 0.00004 < 0.01). As the concentration of ammonia-N increased, AKP generally demonstrated an “induction-suppression” activity. In conclusion, the stress of ammonia-N could influence these immuno-enzymes’ activities.

文章引用:
王程昊, 钱琦凡, 张耀尹, 吴茜丹, 金建钰. 氨氮胁迫对泥蚶免疫酶活性的影响[J]. 水产研究, 2017, 4(3): 65-70. https://doi.org/10.12677/OJFR.2017.43011

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