HJAS  >> Vol. 2 No. 2 (June 2012)

    “绿岭”核桃种胚组培苗腋芽快繁体系的初步研究
    Rapid Propagation of “Lv Ling” Walnut (Juglans Regia L.) through Embryo-Induced Seedlings

  • 全文下载: PDF(766KB) HTML    PP.25-29   DOI: 10.12677/hjas.2012.22005  
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作者:  

刘喜星,李保国,齐国辉,回彦哲,董丽欣,马丽媛:河北农业大学林学院;
顾玉红,刘 璐:河北农业大学生命科学学院

关键词:
核桃核桃属腋芽激素 Walnut; Juglans; Embryo; Axillary Buds; Hormones

摘要:

以早实薄皮核桃绿岭成熟种胚诱导出的腋芽为外植体,MS为基本培养基,通过调节激素种类和浓度配比,初步研究了“绿岭”核桃种胚组培苗腋芽快繁体系。比较试验结果表明:最佳种胚萌发培养基为MS + 蔗糖30 g/L + 琼脂6 g/L;最佳腋芽诱导和增殖培养基为MS + 6-BA 2.0mg/L + NAA 0.2 mg/L + 蔗糖30 g/L + 琼脂6 g/L。生根采用两步生根法:先在DKW + IBA 8.0 mg/L + 蔗糖30 g/L + 琼脂6.0 g/L暗培养20 d,然后转入DKW + 蔗糖30 g/L + 琼脂6 g/L培养基中进行光照培养,生根率为50%。诱导生根的组培苗移栽到大田后成活率为33.3%

The mature embryo induced axillary buds were used as explants to investigate rapid propagation of precocious thin-shelled “Lv Ling” walnut in the MS basic medium with different concentrations of plant growth regulators. The results showed that MS + sucrose 30 g/L + agar6 g/L was the most feasible medium for embryo germination, and MS + 6-BA 2.0 mg/L + NAA 0.2 mg/L + sucrose30 g/L + agar6 g/L was the best for induction and multiplication of the axillary buds in all tested treatments. The 50% of rooting rate was achieved by a two-stage rooting method, namely, the shoots were first cultivated in DKW + IBA 8.0 mg/L + sucrose30 g/L + agar6.0 g/L medium for 20 days under dark condition, then transferred to DKW+ sucrose30 g/L + agar6.0 g/L medium under light condition. After transplanting to the field, 33.3% of the seedlings survived.

 

文章引用:
刘喜星, 顾玉红, 李保国, 齐国辉, 刘璐, 回彦哲, 董丽欣, 马丽媛. “绿岭”核桃种胚组培苗腋芽快繁体系的初步研究[J]. 农业科学, 2012, 2(2): 25-29. http://dx.doi.org/10.12677/hjas.2012.22005

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