采用荧光探针法检测药物中加替沙星
Detection of Gatifloxacin in Drugs by Fluorescence Probe Method
DOI: 10.12677/AAC.2019.93024, PDF,  被引量    国家自然科学基金支持
作者: 赵雅欣, 周子冉, 翟 月, 林 鹏, 程雨晴, 赵 畅, 郭 强, 吕玉光*：佳木斯大学药学院，黑龙江 佳木斯
关键词: 三氰基二氢呋喃；加替沙星；络合物；荧光分光光度法；Tricyanodihydrofuran； Gatifloxacin； Complex； Fluorophotometry
摘要: 通过合成三氰基二氢呋喃(DCDHF-2-V)电子受体，设计了(DCDHF-2-V)荧光探针。创建了一种三氰基二氢呋喃衍生物与加替沙星进行反应形成的多种荷移络合物为其进行含量检测的新方法，同时测定在波长范围内可以达到增加灵敏度，并研究其原理，探讨实验进行的最佳条件。在30℃下反应30 min时，所形成的荷移络合物的荧光强度最高，合成了新型电子受体–新型三氰基二氢呋喃。通过对三氰基二氢呋喃衍生物与加替沙星药物形成的络合物进行荧光探针，开辟了新型药物荧光探针的新思路。通过实验的方式确定了各自最佳的合成条件。研究表明络合物在特定的范围内加替沙星的浓度与荧光强度直接存在一定程度的线性关联，而且络合物的荧光特性明显强于单个加替沙星的荧光特性。
Abstract: A fluorescent probe (DCDHF-2-V) was designed by synthesizing the new three cyanide two hydro-furan (DCDHF-2-V) electron acceptor. A new method of measuring the content of a new type of tricyanodihydrofuran and gatifloxacin was established. At the same time, the sensitivity was in-creased in the wavelength range, and the principle was studied and the best conditions for the ex-periment were discussed. The fluorescence intensity of the charge transfer complex was the highest when it was reacted for 30 minutes at 30˚C, and a new electron acceptor tricyanodihydrofuran was synthesized. A new fluorescence probe for the new three cyanohydrofuran derivatives and gatifloxacin was developed, which opened up a new idea of the new drug fluorescent probe. The best synthesis conditions were determined by experiments. The results show that the concentration of gatifloxacin in a specific range has a linear relationship with the fluorescence intensity, and the fluorescence characteristics of the complex are obviously stronger than that of the single gat-ifloxacin.
文章引用：赵雅欣, 周子冉, 翟月, 林鹏, 程雨晴, 赵畅, 郭强, 吕玉光. 采用荧光探针法检测药物中加替沙星[J]. 分析化学进展, 2019, 9(3): 179-185. https://doi.org/10.12677/AAC.2019.93024

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