不同规格线纹海马氨基酸构成和降压肽的分析研究

Analysis of Amino Acid Ingredient and Antihypertensive Peptides in the Lined Seahorse with Different Specifications

摘要: 线纹海马(Hippocampus erectus)是如今海马养殖业热门品种，因其具有高抗病性、繁殖能力强等优势，逐渐变成了海马养殖业的首选种，具有极高的药用价值和观赏价值。本文以线纹海马为研究对象，通过分析测定不同规格的线纹海马氨基酸构成和海马降压肽对血管紧张素转化酶ACE的抑制率来研究线纹海马制备降压肽的条件。结果表明，高效液相色谱法在料液比为1:15，加酶量为3%，酶解温度为37℃的条件下，酶解时间6 h，商品规格为12 cm以上的线纹海马抑制率最高，制备降压肽效果最好。

Abstract: The lined seahorse (Hippocampus erectus) is a hot variety in the seahorse breeding industry today. Because of its advantages of high disease resistance and strong reproductive ability, it has gradually become the preferred species in the seahorse breeding industry, with extreme medicinal value and ornamental value. In this paper, the lined seahorse was selected as the research object, and the conditions for the preparation of the lined seahorse antihypertensive peptide were studied by analyzing the amino acid composition of the lined seahorse of different specifications and the inhibitory rate of the hippocampal antihypertensive peptide on ACE. The results showed that under the conditions of a solid-liquid ratio of 1:15, an enzyme dosage of 3%, an enzymatic hydrolysis temperature of 37˚C, and an enzymatic hydrolysis time of 6 hours, the inhibition rate of linear hippocampus with a commercial specification of 12 cm or more was the highest, and the effect of preparing antihypertensive peptides was the best.