市售活菌型乳酸菌饮品的润肠通便性能研究

Performance Study on Commercially Available Live Lactobacillus Beverages on the Bowel Regulation

摘要: 目的：探究市面上活菌型乳酸菌饮品在销售过程中的活菌数，以及是否具有调节肠道健康的功能。方法：选取济宁地区广泛销售的三种活菌型乳酸菌饮品(分别为A饮品、B饮品和C饮品)为研究对象，首先检测其保质期内的活菌数，其次用三种饮品分别灌胃小鼠，14天后，以盐酸洛哌丁胺构建小鼠便秘模型，通过测定小鼠小肠墨汁推进率、粪便含水量、血清胃肠调节肽含量和肠组织AQP3和5-HT的表达量等指标，评价三种饮品的润肠通便性能。结果：三种饮品在保质期内活菌数基本不变，其中A饮品活菌数较B和C饮品少。C饮品显著提高便秘小鼠小肠推进率、小鼠血清P物质(SP)含量和肠组织AQP3的表达，而A和B饮品未表现出显著促进作用。结论：不同饮品在活菌数和润肠通便功能方面有所差异，这为我们了解本地活菌型乳酸菌饮品市场和规范其销售提供理论基础。

Abstract: Objective: To investigate the viable bacterial count in commercially available live Lactobacillus beverages during sales and assess their potential intestinal health-regulating functions. Methods: Three popular live Lactobacillus beverages (Brand A, B, and C) widely sold in Jining were selected. Their viable bacterial counts were determined within the shelf life. Mice were administered these beverages via gavage for 14 days. A constipation model was then induced using loperamide hydrochloride. The bowel regulation effects were evaluated by measuring intestinal ink propulsion rate, fecal water content, serum gastrointestinal regulatory peptide levels, and intestinal AQP3 and 5-HT expression. Results: All three beverages maintained stable viable bacterial counts throughout their shelf life, with Brand A exhibiting lower counts than Brands B and C. Brand C significantly enhanced intestinal propulsion rate, serum substance P (SP) levels, and intestinal AQP3 expression in constipated mice, while Brands A and B showed no significant effects. Conclusion: Significant differences exist in viable bacterial counts and bowel regulation efficacy among beverages, which establishes a theoretical basis for understanding the local market of live Lactobacillus beverages and regulating their sales in this region.