匀浆负压空化法提取棕榈藤酚酸类化合物及其光热稳定性研究

Homogenate Negative Pressure Cavitation Extraction of Phenolic Acid from Rattan and Stability Analysis of Being Heated and Being UV Irradiation

摘要: 本研究以棕榈藤叶片为原料，对其进行匀浆负压空化法提取后，分别实验探究了乙醇体积分数、料液比、匀浆时间、空化时间、空化速度和空化仪器形状6方面因素并找出最优操作条件。本文还对匀浆辅助萃取的机理进行了阐释，以及对棕榈藤多酚的光热稳定性进行对照实验分析。实验结果表明：以体积分数60%的乙醇溶液为提取溶剂；料液比1:14 g/mL；匀浆210 s后；加入到梨形空化设备中，空气流速为200 mL/min，空化时间为70 min；在此最佳匀浆负压空化的条件下，所得棕榈藤叶片中总酚酸的提取率为4.32 mg/g。棕榈藤酚酸提取物的光热稳定性分析表明：低于50℃时热稳定性较好，在90℃受热12 h，总酚酸含量降解25%；光稳定性较好，室内自然光照射12 h内的含量基本不变，稳定性优于在UVA和UVB紫外照射条件下对酚酸类化合物含量稳定性的影响。

Abstract: In this study, Rattan (palm cane) was extracted by homogenate-assisted extraction as raw material. A research has been conducted about ethanol volume fraction, feed liquid ratio, homogenate time, cavitation time, cavitation velocity, and the instrument shape in order to find out the optimum parameters. Moreover, the mechanism of homogenate-assisted extraction was elucidated and the stability analysis for being heated and being UV irradiation was studied. The experimental results showed that the extraction solvent is 60% ethanol solution; feed liquid ratio is 1:14 g/mL; homogenate time is 210s; cavitation speed is 200 mL/min, and cavitation time is 70min; instru-ment shape is pyriform shape. Under the optimum conditions, the extraction yield of total phenolic acid from Rattan is 4.32 mg/g. The total phenolic acid showed a better stability below 50˚C. The content of total phenolic acid was degraded 25% after being heated at 90˚C for 12h. It had a better stability with being natural light irradiation than the stability with being UVA and UVB irradiation.