滇重楼遗传多样性的AFLP分析
AFLP Analysis on Genetic Diversity of P. polyphylla var. yunnanensis
DOI: 10.12677/BR.2018.71007, PDF,  被引量    国家自然科学基金支持
作者: 李艳冰, 刘 涛*：云南农业大学农学与生物技术学院，云南，昆明；王 玲, 杨生超：西南中药材种质创新与利用国家地方联合工程研究中心，云南，昆明；何忠俊, 谢世清, 廖秋红：云南省优势中药材规范化种植工程研究中心，云南，昆明
关键词: 滇重楼；种质资源；遗传多样性；AFLP；P. polyphylla var. yunnanensis； Germplasm Resources； Genetic Diversity； Amplified Fragment Length Polymorphism (AFLP)
摘要: 目的：探索云南滇重楼(Paris polyphylla var. yunnanensis)种质资源的遗传多样性，为滇重楼种质资源保护、亲缘关系研究、品种选育和引种栽培提供分子生物学依据。方法：利用AFLP 标记对来自云南不同产地的15份滇重楼开展遗传多样性分析，采用NTSYS软件进行聚类和主坐标分析。结果：在物种水平上，多态位点百分率(PPL)为48.25%，Shannon信息指数(I)为0.3903，Nei’s基因多样性指数平均为0.2768群体总的遗传多样性(Ht)为0.2519，而居群内的遗传多样性(Hs)为0.1868，群体间遗传分化系数Gst为0.2583，即居群间的遗传变异占总变异的25.84%，而居群内的遗传变异为74.17%。结论：AFLP标记的结果表明滇重楼的遗传多样性程度较低，群体间分化程度很低。居群间基因流(Nm)为0.7178，表明居群之间遗传交换较少。
Abstract: Objective: To evaluate the genetic diversity of germplasm resources for P. polyphylla var. yunna-nensis and provide biological reference for germplasm resources protection, genetic relationship analysis, breeding, and introduction and cultivation of P. polyphylla var. yunnanensis. Methods: Amplified fragment length polymorphism (AFLP) marker was developed to analyze the genetic diversity among 15 samples of P. polyphylla var. yunnanensis from various habitats in Yunnan re-gion, and cluster analysis and principal coordinate analysis were revealed by the NTSYS software. Results: At the species level, the percentage of polymorphic loci (PPL) was 88.15%. Shannon’s in-formation index (I) was 0.4158. Nei’s genetic diversity index was 0.2533. The total genetic diversity (Ht) was 0.2519, the intra-population genetic diversity (Hs) was 0.1868 and the inter-popu- lation genetic differentiation (Gst) was 0.2583. Conclusion: The results implied the variation was mainly from intra-population while the degree of differentiation in inter-populations was small. The gene flow (Nm) between group is 0.7178 which shows that the genetic exchange between group is less.
文章引用：李艳冰, 王玲, 何忠俊, 谢世清, 廖秋红, 杨生超, 刘涛. 滇重楼遗传多样性的AFLP分析[J]. 植物学研究, 2018, 7(1): 45-53. https://doi.org/10.12677/BR.2018.71007

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