基于全二维气相色谱在线SPE技术分析沉香精油挥发性香气成分
Analysis of Components in Agarwood Essential Oil by On-Line Solid Phase Extraction with Comprehensive Two-Dimensional Gas Chromatography-Mass Spectrometry
DOI: 10.12677/BR.2018.74047, PDF,  被引量    科研立项经费支持
作者: 皮立, 谭亮, 肖远灿, 迟晓峰, 董琦, 陈涛, 李玉林*：中国科学院西北高原生物研究所，中国科学院藏药研究重点实验室，青海 西宁；王正逊：西安睿德安电子科技有限公司，陕西 西安
关键词: 沉香精油；全二维气相色谱；挥发性成分；在线固相萃取；倍半萜化合物；Agarwood Essential Oil； Comprehensive Two-Dimensional Gas Chromatography； Volatile Components； Online Solid Phase Extraction (Online SPE)； Sesquiterpenes Compounds
摘要: 采用一维气相色谱–质谱法(1DGC-MS)和全二维气质联用在线SPE装置(GC-SPE-GC-MS)分析了沉香精油的组成情况，对部分常见物质进行了定性分析。结果表明，对于同一沉香精油样品，1DGC-MS只鉴定出样品中匹配度大于800的共45种化合物，分别为萜烯类7种、醇类17种、酯类4种、酮类6种、醛类4种、酸和其他杂环烃类7种；GC-SPE-GC-MS装置结合标准谱图辅助定性和手动扣除，定性出匹配度大于800的共95种化合物，分别为萜烯类10种、醇类27种、酯类12种、醛类6种、酮类14种、其他杂环和饱和长链长链烃类等26种。研究表明以苄基丙酮、香柏酮、愈创木醇、沉香呋喃、桉叶醇、沉香螺旋醇、苍术醇、白木香醛8种特性组分可以作为沉香精油的质量评价指标。
Abstract: For analysis of composition of perfumery product, two detection technologies, one-dimensional gas chromatography-mass spectrometry (1DGC-MS) and On-line SPE with comprehensive two-dimensional gas chromatography-mass spectrometry (GC-SPE-GC-MS) were applied. Conven-tional components in perfumery product were identified and classified. Results showed that 1DGC-MS can identify 45 compounds with high matched results with MF > 800 when matched with dedicated standard library. Among these compounds, they are constituted of 7 terpenes, 17 alco-hols, 4 esters, 6 ketones, 4 aldehydes, 7 acids and other heterocyclic compounds. While by the help of automatic processing of software and manually deduct background, GC-SPE-GC -MS can identify 95 compounds with matched results MF > 800, and those compounds are classified 10 terpenes, 27 alcohols, 12 esters, 14 ketones, 6 aldehydes, 26 heterocyclic and alkane compounds. The inves-tigation showed that the eight characteristic components, including 4-phenyl-2-butanone, agaros-pirol, agarofuran, nootkatone, guaiol, eudesmol, hinesol and baimuxinal, could be used as the quality evaluation index of Agarwoods essential oil. 
文章引用：皮立, 王正逊, 谭亮, 肖远灿, 迟晓峰, 董琦, 陈涛, 李玉林. 基于全二维气相色谱在线SPE技术分析沉香精油挥发性香气成分[J]. 植物学研究, 2018, 7(4): 386-397. https://doi.org/10.12677/BR.2018.74047

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