龙葵组织培养快繁体系的建立

Establishment of Tissue Culture Rapid Propagation System for Solanum nigrum

摘要: 以龙葵无菌苗茎段和叶片为外植体，通过不定芽的诱导及生根培养，筛选出最佳诱导培养基。结果表明：茎段最佳诱导培养基为MS + NAA 0.5 mg/L + 6-BA 2.0 mg/L，其诱导率为92.5%；叶片最佳诱导培养基为MS + NAA 0.1 mg/L + 6-BA 2.0 mg/L，诱导率高达100%；最佳生根培养基为1/2MS + NAA 0.5 mg/L，炼苗移栽成活率98%。结论：通过对龙葵组培快繁体系的研究，为实现优质种苗的工厂化生产提供理论依据，以满足大规模生产的需求。

Abstract: The stems and leaves of asparagus seedlings were used as explants, and the optimal induction medium was selected through induction of adventitious buds and rooting culture. The results showed that the best induction medium for stems was MS + NAA 0.5 mg/L + 6-BA 2.0 mg/L, and the induction rate was 92.5%; the optimal induction medium for leaves was MS+NAA 0.1 mg/L + 6-BA 2.0 mg/L, and the induction rate was as high as 100%; the most suitable rooting medium was 1/2MS + NAA 0.5 mg/L, and the survival rate of the transplanted plants was 98%. Conclusion: Through the research on tissue culture rapid propagation of Solanum nigrum, it provides the theoretical basis for the industrial production of high-quality seedlings to meet the requirements of large-scale production.