野生黑果枸杞(Lycium ruthenicum)原花青素与多糖含量的比较分析研究

Comparative Analysis of Proanthocyanidins and Polysaccharides on Wild Lycium ruthenicum

摘要: 本研究选择采集自青海省、内蒙古、宁夏和甘肃省等不同地区的野生黑果枸杞资源为研究对象，测定了供试材料的原花青素和多糖含量等常规指标，比较分析了不同地区供试材料原花青素和多糖含量的差异性及其相关性，并采用类平均距离系统聚类的方法对供试材料进行了类群划分。结果表明，供试材料黑果枸杞果实原花青素吸光度依次顺序为4号 > 1号 > 5号 > 6号 > 3号 > 2号，其中，4号野生黑果枸杞品种花青素吸光度(2.43)显著地高于其他材料(P < 0.05)，2号原花青素吸光值最低，仅为1.35，仅3号与6号间差异不显著(P > 0.05)，其余彼此间均存在显著性差异(P < 0.05)；多糖含量依次为：3号 > 7号 > 2号 > 4号 > 5号 > 6号 > 1号，3号与7号间差异不显著(P > 0.05)，且显著高于其他材料(P < 0.05)。而且原花青素与多糖含量二者变异性明显，但彼此间相关性无一致性规律。从原花青素吸光值而言，供试材料1号与4号可划归为一个类群；其余2号、3号、5号与6号，可归为一个类群，可为引种繁育优良品种提供理论依据。

Abstract: The difference and correlation analysis were analyzed on Proanthocyanidins and Polysaccharide of wild Lycium ruthenicum experimental materials from different regions including Qinghai Province, Gansu Province, Ningxia Autonomous Region & Inner Mongolia. Cluster analysis was also used to classify the experimental materials. The results showed that the order of Proanthocyanidins absorbance in Lycium ruthenicum experimental materials was No. 4 > No. 1 > No. 5 > No. 6 > No. 3 > No. 2. The Proanthocyanidins absorbance of No. 4 (2.43) was significantly higher than that of other materials (P < 0.05). That of No. 2 was the lowest, only 1.35, but there was no significant difference between No. 3 and 6 (P > 0.05). Meanwhile, there was a significant difference between the others (P < 0.05). The content of Polysaccharide was in sequence: No. 3 > No. 7 > No. 2 > No. 4 > No. 5 > No. 6 > No. 1. The difference between No. 3 and 7 was not significant (P > 0.05), and was significantly higher than that of other materials (P < 0.05). Moreover, the variation of Proanthocyanidins and Polysaccharide content was obvious among the experimental materials, but there was no consistency about the correlation analysis between them. From the aspect of Proanthocyanidins, the experimental materials No. 1 and No. 4 could be classified as a group. The remaining No. 2, No. 3, No. 5 and No. 6 belonged to a group. The whole results could provide theoretical basis for introduction and breeding of fine varieties in the future.