石榴皮多酚的分离纯化及抗氧化性研究

Research on Separation, Purification and Antioxidation of Polyphenols from Pomegranate Peel

摘要: 以石榴皮为原料，在单因素试验的基础上用正交试验优化石榴皮多酚提取的工艺条件，利用大孔吸附树脂对石榴皮多酚进行纯化，通过显色反应和紫外光谱分析对纯化后石榴皮多酚进行性质鉴定，并进一步研究了纯化后的石榴皮多酚的抗氧化性。结果表明：石榴皮多酚提取的最优工艺条件是乙醇体积分数70%、料液比1:30 (g/mL)、提取温度55℃、提取时间5 h，提取率为12.68%；DM130树脂纯化石榴皮多酚效果较好，纯化物纯度为90.56%；初步判断纯化物含有酚类物质；大孔吸附树脂纯化物抗氧化性作用较好。

Abstract: Using pomegranate peel as raw material, based on single factor experiment, orthogonal experi-ments were used to optimize the extraction conditions of polyphenols from pomegranate peel. The polyphenols of the pomegranate peel were purified by the macroporous adsorption resin method. The color reaction and UV spectrum analysis were used. The purified pomegranate peel polyphenols were characterized, and the antioxidative properties of the pomegranate peel poly-phenols obtained after purification were further studied. The experimental results showed that the optimal conditions for the extraction of polyphenols from pomegranate peel were as following: ethanol volume fraction 70%, solid-liquid ratio 1:30 (g/mL), extraction temperature 55˚C, extrac-tion time 5 h, extraction rate 12.68%, and DM130 resin. The purified polyphenols from pome-granate peel had good effects, and the purity of the purified product was 90.56%. The purified product contained phenolic substances. The purified macroporous resin had good antioxidant ac-tivity.