六斑刺鲀胚胎发育形态观察

The Observation of Embryo Development Morphology of Diodon holocanthus

摘要: 六斑刺鲀是一种极具养殖开发潜力的经济鱼类，广泛分布于我国东南沿海，肉质鲜美，鱼皮富含丰富的胶原蛋白。本文研究了六斑刺鲀受精卵的胚胎发育过程。刺鲀卵为浮性卵，浮在水面后聚成一片，略有粘性。卵子受精后原生质向动物极集中并逐渐隆起，胚盘形成，约经过20 min第一次分裂开始，以后约每20 min再分裂一次，受精2 h后为多细胞期。受精13 h后进入原肠期，21 h后发育至神经胚期，27 h后进入尾芽期。在盐度28~32，温度28℃~29℃，pH 7.2~8.5实验条件下，六斑刺鲀受精卵经过约83小时便可出膜。这是国内针对六斑刺鲀胚胎发育形态结构变化的首次报道。

Abstract: Abstract Porcupine puffer (Diodon holocanthus) was regarded as a significant commercial fish species with a high economic potential. Meat of them was delicious, and skin was rich in collage protein. Here, we explored the embryonic development of the fertilized eggs of D. holocanthus. The fertilized eggs were slightly sticky and floating egg that came together on the surface of the water. After fer-tilization, the protoplasm was concentrated towards the animal pole and gradually rised, and the blastoderm forms, which started after the first division in about 20 min, and then divided again about every 20 min. After fertilization, it was a multicellular stage after 2 h. After 13 h of fertiliza-tion, it entered the gastral stage, grew to the neuronal embryo stage after 21 h, then entered the tail bud stage after 27 h. Under the condition of this experiment, salinity 28 - 32, temperature 28˚C - 29˚C, pH 7.2 - 8.5, the fertilized egg could be out of the membrane after about 80 hours. This was the first report on the changes of embryonic morphological structure of D. holocanthus.