高效液相色谱指纹图谱法鉴别咖啡掺假
Research on Identification of Adulteration Coffee by HPLC Fingerprints
DOI: 10.12677/AAC.2018.84022, PDF,    科研立项经费支持
作者: 邵仕萍*, 奚星林, 陈远鸿, 黄翠薇：广东检验检疫技术中心，广东 广州；文慧依：广东省动植物与食品进出口技术措施研究重点实验室，广东 广州
关键词: 指纹图谱；咖啡；掺假；高效液相色谱；Fingerprint； Coffee； Adulteration； High Performance Liquid Chromatography
摘要: 通过采集90个纯咖啡样品的高效液相色谱图建立起咖啡的标准指纹图谱，建立了一种基于高效液相色谱指纹图谱鉴别咖啡掺假的方法，利用中药色谱指纹图谱相似度评价系统A版和中药色谱图分析和数据管理系统软件分析待测样品色谱图与咖啡标准指纹图谱的相似度，并根据相似度的分析结果鉴别咖啡是否掺假。结果表明，该方法稳定性好，纯咖啡标准指纹图谱包括17个共有峰，其高效液相色谱指纹图谱峰面积相关系数法、峰面积夹角余弦法、峰高相关系数法、峰高夹角余弦法阈值均大于0.9，模拟掺入大麦粉、黑玉米粉和巴西莓果粉，得到掺假物的峰面积相关系数法、峰面积夹角余弦法、峰高相关系数法、峰高夹角余弦法阈值均小于0.4，且本方法对大麦粉、黑玉米粉和巴西莓果粉可鉴别最小掺假量为1 wt%，具有方便快捷，精密度好，鉴别能力强等特点。
Abstract: A method had been developed for identification of true or false coffee based on high performance liquid chromatography (HPLC) fingerprint. 90 high performance liquid chromatography (HPLC) fingerprints of coffee were selected and analyzed, and all data were calculated by using A version of chromatographic fingerprint similarity evaluation system of Chinese traditional medicine and the chromatographic analysis and data management system of Chinese traditional medicine softwares. In the whole process, the stability of the method was good, the standard fingerprints of pure coffee owned 17characteristic peaks of HPLC fingerprint, the similarity threshold of all 30 samples was no less than 0.9 and the similarity threshold of adulterated coffee was as low as 0.4 through analyzing peak area correlation coefficient (r), peak area cosine ratio (cosα), peak height correlation coefficient (r), peak height cosine ratio (cosα). The method can identify that the minimum adulterated amount of black barley flour, black corn flour and Brazilian berry powder was 1 wt%. Such method was convenient, high-precision and had strong discrimination ability, which was worthy of popularized application.
文章引用：邵仕萍, 奚星林, 陈远鸿, 黄翠薇, 文慧依. 高效液相色谱指纹图谱法鉴别咖啡掺假[J]. 分析化学进展, 2018, 8(4): 181-190. https://doi.org/10.12677/AAC.2018.84022

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