龙胆草三萜化合物的提取工艺研究

Study on Extraction Technology of Triterpenoids from Gentian

摘要: 以龙胆草为原料，采用水浴加热、超声振荡法提取龙胆草中三萜化合物，以紫外光谱法检测其含量。通过乙醇含量、反应温度、反应时间、料液比等单因素试验和正交试验，得到最佳提取工艺。试验结果表明，最佳提取工艺为反应温度65℃，乙醇含量80%，料液比1:35 g/mL，反应时间2.5 h，在此条件下测得三萜化合物提取率为1.578%。

Abstract: Triterpenoids from gentian is extracted in combination with ultrasound and determined by UV spectrum. The best extraction process was obtained by single factor test and orthogonal test such as ethanol concentration, reaction temperature, solid-liquid ratio, and extracting time. The results suggest that the optimum conditions for this extraction are ethanol concentration 80%, reaction temperature 65˚C, solid-liquid ratio 1:35 g/mL, extracting time 2.5 h. Under the optimum condition, the extraction rate of triterpenoids is 1.579%.