野生红托竹荪菌株分离驯化与区别性鉴定

Isolation, Domestication and Differential Identification of Wild Dictyophora rubrovalvata Zang, ji et liou

摘要: 采集异地野生红托竹荪8 ~ 9成熟菌蛋子实体，分离驯化母种、栽培种，察看农艺性状，对栽培种作区别性鉴定实验。结果：驯化培育栽培种菌丝洁白、浓密，长势旺盛；对峙培养栽培种，20天左右，不同栽培种菌落菌丝相遇互不相让，形成一道加深隔离线，产生拮抗反应。相同栽培种菌落菌丝融合在一起，没有发生拮抗反应。结论：拮抗实验操作简单，用时短，可以区分红托竹荪不同菌种。

Abstract: In this paper, the eggs of 8 - 9 mature Dictyophora rubrovalvata Zang, ji et liou were collected, the mother species were isolated and domesticated, cultivated species were cultivated, agronomic characters were observed, and the differentiation of cultivated species was carried out. Results: The mycelium of cultivated species was white, dense and vigorous, and about 20 days after cultivation, the colony mycelium of different cultivated species met with each other, forming a deep isolation line and antagonistic reaction. The mycelium of the same cultivated species was fused together and there was no antagonistic reaction. Conclusion: The antagonistic experiment is simple in operation and short in time, and it can be used to distinguish different strains of Dictyophora rubrovalvata Zang, ji et liou.