等离子体处理鲍曼不动杆菌生物膜的研究

Study on Plasma Treatment of Acinetobacter baumannii Biofilm

摘要: 研究了高压脉冲等离子体射流(APPJ)对耐药性鲍曼不动杆菌(A. baumannii)生物膜的失活效果和机制。研究发现，该脉冲等离子体射流对生物膜中的细菌有明显的灭活作用。菌落形成单位(CFU)计数方法处理显示，30分钟处理后，生物膜中99.9%的细菌失去其可培养发育能力。与此同时，刃天青荧光染色方法显示约80%的细菌失去了新陈代谢能力，部分细菌进入了一种存活但不可培养发育的状态(VBNC)，H₂-DCFDA荧光染色方法显示，等离子体处理后生物膜中细菌内的活性含氧基团(ROS)的浓度有所提高。等离子体活性物种可能诱导了细菌内部应激反应的产生，导致胞内ROS的生成，最终氧化破坏细菌结构，这可能是等离子体导致生物膜中细菌失活的重要原因。

Abstract: The inactivation effect and mechanism of high atmospheric pressure pulsed plasma jet (APPJ) on drug-resistant Acinetobacter baumannii (A. baumannii) Biofilm were studied in this paper. It is found that the pulsed plasma has a remarkable ability to inactivate bacteria in biofilm. The CFU counting method of colony forming units showed that almost all the bacteria (99.9%) in the biofilm lost their culturability after 30 minutes of treatment. At the same time, about 80% of the bacteria had lost their metabolism, and some of the bacteria had entered a viable but uncultured state of VBNC. H₂-DCFDA fluorescence staining showed that the concentration of active oxygen-containing group (ROS) in biofilm was increased after plasma treatment. Plasma active species may induce internal stress reaction of bacteria, lead to a large number of intracellular ROS production, and ultimately oxidize the bacterial structure, which may be an important reason for the inactivation of bacteria in biofilm caused by plasma.