胎盘生长因子在下肢动脉斑块中表达的相关性研究

Correlation of Placental Growth Factor Expression in Arterial Plaque of Lower Extremity

摘要: 目的：探讨人股动脉粥样硬化斑块中胎盘生长因子表达水平及临床意义。希望进一步探讨下肢动脉疾病的发病机制。方法：研究共纳入下肢动脉斑块标本30例(其中男性15例，女性15例，平均年龄：66岁)，所有病例术前均有间歇性跛行病史，术前均经下肢动脉CTA或血管造影等检查，证实为下肢动脉硬化闭塞症。同时选取因外伤损伤股动脉后，行股动脉搭桥术中切除的股动脉标本10例(男性7例，女性3例，平均年龄：61岁)作为对照组。采用免疫组化方法检测PLGF蛋白表达含量水平。结果“各组PLGF水平比较((正常对照组(0.0005 ± 1.52 × 10⁻⁷) vs.动脉斑块组(0.0388 ± 1.1 × 10⁻³)，P < 0. 05)，差异有统计学意义。结论：我们的研究发现，下肢动脉疾病患者动脉斑块PLGF水平明显高于正常对照者，这可能与PLGF可以促进动脉粥样硬化斑块的增长和导致斑块不稳定有关。

Abstract: Objective: To investigate the expression of placental growth factor in human femoral atherosclerotic plaque and its clinical significance. We hope to further explore the pathogenesis of ASO. Methods: A total of 30 lower extremity arterial plaque specimens (15 males and 15 females, mean age: 66 years) were included in the study. All patients had a history of intermittent claudication before operation. All patients were confirmed as arteriosclerosis obliterans by lower extremity CTA or angiography before operation. Meanwhile, 10 cases (7 males and 3 females, mean age: 61) of femoral artery specimens removed during femoral artery bypass grafting after femoral artery injury were selected as the control group. The expression of PLGF protein was detected by immunohistochemistry. Result: The PLGF levels of each group were significantly different from those of the normal control group ((0.0005 ± 1.52 × 10⁻⁷) vs. the arterial plaque group (0.0388 ± 1.1 × 10⁻³), P < 0.05). Conclusion: Our study found that PLGF levels in lower extremity artery disease patients are much higher than those in normal controls, which may be related to the role of PLGF in promoting the growth of atherosclerotic plaques and leading to plaque instability.