沙田柚自交不亲和花柱数字基因表达谱及相关基因分析

Digital Gene Expression Profiling Analysis of Self-Incompatibility Styles in Citrus grandis var. Shatinyu

摘要: 为探索沙田柚自交不亲和的分子机理，以沙田柚自交与异交1~3天的花柱为材料，利用数字基因表达谱技术对样品cDNA文库进行差异基因表达谱分析。结果表明：异交1 d/自交1 d花柱中，上调表达的基因有244个，下调表达的基因有27个；异交2 d/自交2 d花柱中，上调表达基因有265个，下调表达基因有244个；异交3 d/自交3 d花柱，上调表达的基因有193个，下调表达的基因有685个。在SY1/SZ1、SY2/SZ2和SY3/SZ3中共有的差异表达的基因为30个。差异表达基因的GO功能显著性富集分析结果表明，这些差异表达的基因主要参与应对各种化学物质、植物激素、内源和外源物质的刺激及信号转导过程。通过本研究，挖掘出了一些与沙田柚自交不亲和相关的基因，可为今后阐明沙田柚配子体自交不亲和的分子机理提供依据。

Abstract: This study aimed to understand the self-incompatibility mechanism in Citrus grandis var. Sha-tinyu. The one day, two days and three days styles of Shatinyu after self-pollination and cross- pollination were used to construct DNA libraries and the gene expression profiles were analyzed by Solexa sequencing technology. The results of solexa sequencing showed that a total of 271 different expression genes (DEGs) were obtained from the 1 d self-and cross-pollinated styles, in which 244 up-regulated expression and 27 down-regulated expression. 265 up-regulated ex-pressed genes and 244 down-regulated expressed genes were in 2 d self-and cross-pollinated styles, 193 up-regulated expressed genes and 685 down-regulated expressed genes in compar-ing 3 d self-and cross-pollinated styles. Moreover, statistics of differentially expressed genes showed that there were 30 differentially expressed genes coexisted in SY1/SZ1, SY2/SZ2 and SY3/SZ3. Gene Ontology analysis showed that the DEGs were mainly involved in responding to all kinds of chemicals, plant hormones, the stimulation of endogenous and exogenous sub-stances and signal transduction process. In sum, through this study some genes associated with self-incompatibility in Shatianyu were discovered. These results will provide a theoretical ref-erence for further exploration of self-incompatibility molecular mechanism in Citrus grandis var. Shatinyu.