紫外分光光度法测定望江南种子中总蒽醌含量

Determination of Total Anthraquinones in Seeds of Senna occidentalis by Ultraviolet Spectrophotometry

摘要: 望江南是我国民间常用中药植物，蒽醌是其种子中的主要成分。因紫外分光光度法仪器操作简单，检测速度快，成本低而常常被使用。实验用标准品1,8-二羟基蒽醌为材料，紫外分光光度法检测1,8-二羟基蒽醌的入射光波长，绘制标准曲线，确定线性回归方程，为望江南中总蒽醌的提取工艺研究提供计算依据。结果显示：1,8-二羟基蒽醌的入射光波长为512 nm，线性回归方程为Y = 4.4663X + 0.0093，相关系数R² = 0.9963，线性关系较好。方法学考察结果也显示该方法是简单稳定、重复性较好，回收率高，测定结果准确，操作简便，可作为望江南种子中总蒽醌含量测定的一种方法。

Abstract: Senna occidentalis is a commonly used traditional Chinese medicine plant in China, and anthraquinone is the main ingredient in its seeds. The UV spectrophotometer is often used because of its simple operation, fast detection speed and low cost. The experiment uses standard 1,8-dihydroxyanthraquinone as the material, detects the incident light wavelength of 1,8-dihydroxyanthraquinone by Ultraviolet Spectrophotometry, draws a standard curve, determines the linear regression equation, and provide calculation basis for the research on the extraction process of total anthraquinone in seeds of S. occidentalis. The results show that the incident light wavelength of 1,8-dihydroxyanthraquinone is 512 nm, the linear regression equation is Y = 4.4663X + 0.0093, the correlation coefficient R² = 0.9963, and the linear relationship is good. The methodological investigation results also show that the method is simple, stable, good reproducible, high recovery rate, accurate and easy to operate. It can be used for the determination of total anthraquinone content in S. occidentalis seeds.