针刺对5-HT₇R跨膜信号通路的影响与其改善PCPA失眠中枢5-HT障碍机制的关联性探讨

Study the Acupuncture Effect on 5-HT₇R Transmembrane Signaling Pathway and the Exploration of the Relationship between This Effect and the Acupuncture Improving the Pathogenesis of Central 5-HT Transmitter Disorder in PCPA Insomnia

摘要: 目的：观察针刺5-HT₇R跨膜信号通路的影响，探讨其与改善PCPA失眠大鼠中枢5-HT障碍机制的关系。方法：64只大鼠经随机、PCPA失眠造模及筛选后，共分4组各16只；正常组、模型组无特殊处理，针刺组给予针刺失眠方合脐内环穴干预，非穴组予针刺非穴处理，治疗6天后分离大鼠海马匀浆，ELISA法检测5-HT₇R和cAMP含量，免疫印迹检测Gαs表达；统计学分析5-HT₇R与cAMP相关性。结果：PCPA模型大鼠海马5-HT₇R和cAMP明显降低，针刺治疗组5-HT₇R和cAMP含量均显著升高；各组二指标间呈显著正相关性；四组间Gαs相对表达无统计学差异。结论：针刺调控5-HT₇R/Gαs/cAMP信号通路起到改善PCPA失眠作用，这种改善与中枢5-HT递质障碍机制有一定的相关性。

Abstract: Objective: To observe the effects of the acupuncture of combined method of the acupoint-prescription of umbilical inner acupoints and the insomnia acupoint-prescription on 5-HT₇R transmembrane signal pathway and explore its relationship with improving the mechanism of central 5-HT disorder in PCPA insomnia model rats. Methods: After being randomly divided, modeled and screened for PCPA insomnia model, 64 rats were divided into four groups, and each group was 16. The normal group and the model group were given no special treatment, the acupuncture observation group was acupunctured the combined acupoints of the acupoint-prescription of umbilical inner acupoints and the insomnia acupoint-prescription. The non-acupoint group was dealed with acupuncture non-acupoint. After six days of treatment, in the hippocampus homogenates of the rats, the content levels of 5-HT₇R and cAMP were detected by enzyme-linked immunoassay, and their correlation was statistically analyzed. The expression of Gαs was detected by western blot. Results: The contents of 5-HT₇R and cAMP in hippocampal of PCPA insomnia model rats in control group were significantly reduced, while that of the acupuncture treatment group was significantly increased, and there was a significantly positive correlation between them in each group. The relative expression of Gαs in hippocampus was no statistical difference among the four groups. Conclusion: The cupuncture method could improve PCPA insomnia by regulating the 5-HT₇R/Gαs/cAMP signaling pathway. This regulating mechanism is related to the acupuncture method improving the pathogenesis of central 5-HT transmitter disorder of insomnia.