悬浮固相微萃取紫外分光光度法测定黄酒中的苯甲酸
Determination of Benzoic Acid in Yellow Rice Wine by Suspension Solid Phase Microextraction with Ultraviolet Spectrophotometry
摘要: 研究了使用悬浮固相微萃取的方法辅助十二醇对黄酒中的苯甲酸进行萃取的工艺条件，以黄酒中苯甲酸含量为指标，以简化国标中对苯甲酸的萃取与测定步骤为目的，基于十二醇为脂溶性化合物，难溶于水，易于和黄酒样品分离以及十二醇具有较好的富集能力，通过对国标萃取流程进行改进来缩短实验所需的时间，最终以实现通过此方法进行一些样品中苯甲酸含量测定的课程实验的目的，通过单因素实验优化提取工艺，结果表明最优的萃取工艺为涡旋时间34 s，十二醇加入量470 μL，盐加入量1%，在此工艺条件下得到的黄酒中苯甲酸的含量为0.1183 μg/mg。
Abstract: Was studied using the method of suspended solid phase microextraction auxiliary lauryl alcohol technological condi-tions of extracting rice wine of benzoic acid in acid content in yellow rice wine as an index, in order to simplify the extraction and determination of benzoic acid in gb steps for the purpose, based on the lauryl alcohol as the fat-soluble compounds, poorly soluble in water, easily and rice wine sample separation and enrichment of lauryl alcohol has good ability, Through to the national standard ex-traction process was improved to shorten the time needed for the experiment, finally to achieve some samples by this method for the determination of benzoic acid content in the purpose of course experiment, through the single factor experiment to optimize the extraction process, the results show that the optimum extraction process for vortex time 34 s, 470 μL for the amount of lauryl al-cohol, 1% for the amount of salt, The content of benzoic acid in rice wine was 0.1165 μg/mg.
文章引用：章若禛, 饶桂维, 童建颖. 悬浮固相微萃取紫外分光光度法测定黄酒中的苯甲酸[J]. 食品与营养科学, 2021, 10(3): 224-233. https://doi.org/10.12677/HJFNS.2021.103026

参考文献

[1] 肖潇, 李国辉, 雍凌, 等. 黄酒中苯甲酸本底含量与溯源分析及初步风险评估[J]. 中国食品卫生杂志, 2020, 32(3): 306-311.
[2] 中华人民共和国国家卫生和计划生育委员会. 食品安全国家标准食品添加剂使用标准GB2760-2014[S]. 北京: 中国标准出版社, 2014.
[3] Leuthardt, F. (1977) Intermeditirstqffwechsel. Walter de Gruy-ter, Berlin, Germany, 505+657.
[4] 张旭伟, 马振兴, 马勇. 食品中苯甲酸检测新技术的现状探寻[J]. 现代食品, 2020(23): 132-133+136.
[5] 陈梦轩, 吴友谊, 周韫璐, 许梦琪. 基于低共熔溶剂的涡旋辅助悬浮固化-分散液液微萃取/高效液相色谱法测定水中三氯生与三氯卡班[J]. 分析测试学报, 2020, 39(4): 507-513.
[6] 付博, 张吉苹, 姜晖, 周璐. 基于脂肪酸的漂浮液滴固化分散液液微萃取-高效液相色谱法联用测定酒类样品中的4-乙基苯酚和4-乙基愈创木酚[J]. 色谱, 2016, 34(9): 895-900.
[7] 中华人民共和国国家卫生和计划生育委员会国家食品药品监督管理总局. 食品安全国家标准食品中苯甲酸、山梨酸和糖精钠的测定GB5009.28-2016 [S]. 北京: 中国标准出版社, 2016.
[8] 殷德荣. 毛细管柱气相色谱法测定葡萄酒和黄酒中的山梨酸、苯甲酸[J]. 中国卫生检验杂志, 2015, 25(24): 4366-4368.
[9] Leong, M.I. and Huang, S.D. (2008) Dispersive Liquid-Liquid Microextraction Method Based on Solidification of Floating Organic Drop Combined with Gas Chromatography with Electron-Capture or Mass Spectrometry Detection. Journal of Chromatography A, 1211, 8-12. [Google Scholar] [CrossRef] [PubMed]
[10] Zhang, Z., Yang, M.J. and Pawliszyn, J. (1994) Solid-Phase Microextraction. A Solvent-Free Alternative for Sample Preparation. Analytical Chemistry, 66, 844A-853A. [Google Scholar] [CrossRef] 
[11] Pawliszyn, J. (1995) New Directions in Sample Preparation for Analysis of Arganic Compounds. TrAC Trends in Analytical Chemistry, 14, 113-122. [Google Scholar] [CrossRef] 
[12] 李小兰, 李英堂, 孟冬玲, 杨亚玲. 离子液体双水相萃取测定卷烟烟气中酚类物质的研究[J]. 云南大学学报(自然科学版), 2014, 36(4): 571-577.
[13] 邵圣娟, 卫静莉. 聚乙二醇-硫酸铵双水相体系萃取松花粉中总黄酮的工艺研究[J]. 食品工业科技, 2017, 38(11): 266-269+275.
[14] Wu, C.X., Liu, N., Wu, Q.H., et al. (2010) Application of Ultrasound-Assisted Surfactant-Enhanced Emulsification Microextraction for the Determination of Some Organophosphorus Pesticides in Water Samples. Analtica Chimica Acta, 679, 56-62. [Google Scholar] [CrossRef] [PubMed]
[15] Marco, G.-V., Gaurav, R., Brijesh, T., et al. (2018) Extraction and Yield Optimisation of Fucose, Glucans and Associated Antioxidant Activities from Laminaria Digitata by Applying Response Surface Methodology to High Intensity Ultrasound-Assisted Extraction. Marine Drugs, 16, 257. [Google Scholar] [CrossRef] [PubMed]