中亚沙棘 × 中国沙棘杂交F1代果实的主要酚类物质测试分析

Test Analysis of Main Phenolic Substances in F1 Hybrid Fruits of Hippophae rhamnoides ssp. turkestanica-sinensis

摘要: 为了探明中亚沙棘与中国沙棘杂交F1代果实的主要酚类物质，对位于内蒙古鄂尔多斯的10个优株取样测定了果实的黄酮化合物、多酚和花青素，结果表明：双中杂雌沙棘优株鲜全果的6种糖苷和苷元含量，以异鼠李素3-O-芸香糖苷平均含量最高，达129.75 ± 51.16 μg/g，位列第一；有3个优株(占30%)果实中未检测出槲皮素来；10个优株中以“双中杂雌沙棘04号”的黄酮化合物含量最高。双中杂雌沙棘鲜全果所含黄酮化合物含量明显高于蒙中杂雌沙棘和蒙古沙棘。双中杂雌沙棘的鲜籽中所含多酚的平均值最高，达2.60% ± 0.30%，为鲜全果含量0.74% ± 0.14%的3.5倍、鲜果肉含量0.50% ± 0.13%的5.2倍；鲜全果多酚含量以“双中杂雌沙棘04号”最高。所有10个双中杂雌沙棘优株鲜果肉均不含花青素，还有3个优株的鲜果肉、鲜籽部位均不含花青素，其余7个优株的鲜全果总花青素含量平均值为0.00334 ± 0.00139 mg/100g，且以“双中杂雌沙棘05号”的鲜全果总花青素含量为最高。双中杂雌沙棘的有关研究，将为我国沙棘资源建设和开发利用工作起到十分重要的推动作用。

Abstract: In order to find out the main phenolic substances in the F1 generation of the hybrid between H. rhamnoides ssp. turkestanica and H. r. ssp. sinensis (hereafter as Hippophae rhamnoides ssp. turke-stanica-sinensis), 10 superior plants located in Ordos, Inner Mongolia were sampled and deter-mined for testing flavonoids, polyphenols and anthocyanins. The results showed that the average content of isorhamnetin 3-O-rutoside was 129.75 ± 51.16 μg/g, ranking first, in the contents of six glycosides and aglycones in the whole fresh fruit of Hippophae rhamnoides ssp. turkestanica-sinensis, and quercetin was not detected in the fresh fruits of 3 superior plants (30%), and “Shuang-zhongzacishaji 04” had the highest content of flavonoids among the 10 superior plants. The content of flavonoids in Hippophae rhamnoides ssp. turkestanica-sinensis was significantly higher than that in H. rhamnoides ssp. mongolica-sinensis. and H. r. ssp. mongolica. The average value of polyphenols in the fresh seeds of Hippophae rhamnoides ssp. turkestanica-sinensis was the highest, up to 2.60% ± 0.30%, which was 3.5 times of the total fresh fruit content of 0.74% ± 0.14% and 5.2 times of the fresh pulp content of 0.50% ± 0.13%. The content of polyphenols in total fresh fruit was the highest in “Shuangzhongzacishaji 04”. The fresh pulp of all 10 superior plants of Hippophae rhamnoides ssp. turkestanica-sinensis did not contain anthocyanins, and the fresh pulps and seeds of three superior plants did not contain anthocyanins. The average content of total anthocyanins in the total fresh fruit of the other 7 superior plants was 0.00334 ± 0.00139 mg/100g, and the content of total an-thocyanins in the total fresh fruit of “Shuangzhongzacishaji 05” was the highest. The research on Hippophae rhamnoides ssp. turkestanica-sinensis will play a very important role in promoting the construction, development and utilization of Hippophae rhamnoides resources in China.