印记基因H19单核苷酸多态性与不明原因复发性流产的相关性研究

Correlation Study between Genomic Imprinting H19 Single Nucleotide Polymorphism and Unexplained Recurrent Spontaneous Abortion

摘要: 目的：探讨印记基因H19单核苷酸多态性(SNP)与不明原因复发性流产(URSA)发生的相关性。方法：随机选取2010年1月至2021年3月本院34例不明原因复发性流产患者绒毛组织和42例正常流产患者的绒毛组织为研究对象，提取研究对象基因组DNA，SNP测序分析URSA组与对照组患者H19多个SNP位点的差异，分析单核苷酸多态性与疾病的关系。结果：URSA组和对照组的H19基因rs217727位点基因型、等位基因的分布情况差异有统计学意义(p < 0.05)；H19基因rs2107425、rs3741219位点基因型、等位基因的分布情况差异无统计学意义(p > 0.05)。结论：H19基因rs217727位点单核苷酸多态性可能与URSA相关。

Abstract: Objective: To investigate the correlation between imprinted gene H19 single nucleotide polymorphism (SNP) and the occurrence of unexplained recurrent spontaneous abortion (URSA). Methods: Chorionic villus tissues of 34 patients with unexplained recurrent spontaneous abortion and 42 patients with normal miscarriage in our hospital from January 2010 to March 2021 were randomly selected for the study, and the genomic DNA of the study subjects was extracted and SNP sequencing was analyzed. SNP sequencing was used to analyze the differences of H19 SNP loci between URSA group and control group, and to analyze the relationship between single nucleotide polymorphism and disease. Results: The differences in genotype and allele distribution of H19 gene rs217727 locus between URSA group and control group were statistically significant (p < 0.05); the differences in genotype and allele distribution of H19 gene rs2107425 and rs3741219 locus were not statistically significant (p > 0.05). Conclusion: H19 gene rs217727 locus single nucleotide polymorphism may be associated with URSA.