白芍药材及其配方颗粒的UPLC指纹图谱研究
Study on UPLC Fingerprint of Paeonia lactiflora Pall. and Its Formula Granules
摘要: 目的：建立白芍药材及其配方颗粒的UPLC指纹图谱。方法：色谱柱为ACQUITY UPLC BEH C18 (2.1 mm × 100 mm, 1.7 μm)，以0.05%磷酸水溶液–乙腈为流动相，梯度洗脱，流速为0.1 mL/min；检测波长为254 nm；柱温为30℃；采用中药色谱指纹图谱评价系统进行相似度分析。结果：20批白芍药材及其配方颗粒的UPLC指纹图谱共确认11个共有峰，并指认出芍药苷和没食子酸两种化学成分。结论：该方法准确、可行、重现性好，反映白芍药材及配方颗粒多成分的整体面貌，可为白芍药材及其配方颗粒质量评价提供参考。
Abstract:
Objective: To establish the UPLC fingerprint of Paeonia lactiflora Pall. and its formula granules. Methods: The chromatographic column is ACQUITY UPLC BEH C18 (2.1 mm × 100 mm, 1.7 μm) with acetonitrile-0.05% phosphoric acid aqueous solution as the mobile phase in a gradient mode at the flow rate of 0.1 mL/min. The detection wavelength is 254 nm and the column temperature is 30˚C. The similarity is evaluated using a traditional Chinese medicine chromatographic fingerprint eval-uation system. Results: A total of 11 common peaks were identified in the UPLC fingerprint of 20 batches of Paeonia lactiflora Pall. and their formula granules, and two chemical components, pae-oniflorin and gallic acid, were identified. Conclusion: This method is accurate, feasible, and has good reproducibility, reflecting the overall appearance of multiple components in Paeonia lactiflora Pall. and its formula granules. It can provide a reference for the quality evaluation of the Paeonia lactiflora Pall. and its formula granules.
参考文献
|
[1]
|
吴玲芳, 王晓晴, 陈香茗, 等. 白芍化学成分及药理作用研究进展[J]. 国际药学研究杂志, 2020, 47(3): 175-187.
|
|
[2]
|
陈琪, 何祥玉, 周曼佳, 等. 白芍的化学成分、药理作用和临床应用研究进展[J]. 临床医学研究与实践, 2021, 6(11): 187-189.
|
|
[3]
|
吴丽, 王丽丽, 费文婷, 等. 芍药苷和芍药内酯苷对小鼠疼痛模型的镇痛作用及对β-EP、PGE2的影响[J]. 中华中医药杂志, 2018, 33(3): 915-918.
|
|
[4]
|
刘俊彤, 李轶聪, 董丽强, 等. 芍药苷对抗炎反应因子TNF-a和IL6的作用研究[J]. 中西医结合心血管病电子杂志, 2020, 8(30): 1, 6.
|
|
[5]
|
刘玲, 赵建龙. 芍药苷对脂多糖诱导的小鼠急性肝损伤的保护作用[J]. 中国临床药理学杂志, 2016, 32(5): 433-436.
|
|
[6]
|
王蕾, 郑昌吉, 崔然吉. 芍药苷作为抗抑郁药的神经保护作用机制[J]. 人参研究, 2023(1): 29-31.
|
|
[7]
|
高卓维, 陈广鸿, 符路娣. 芍药苷抑制Src/STAT3信号通路协同替莫唑胺抑制胶质瘤细胞增殖和迁移[J]. 实用医学杂志, 2020, 36(23): 3199-3205.
|
|
[8]
|
国家药典委员会. 中华人民共和国药典: 一部[M]. 北京: 中国医药科技出版社, 2020: 108.
|
|
[9]
|
孙叶芬, 岳倩侠, 杜倩倩, 等. 基于质量标志物的白芍配方颗粒量值传递规律及工艺优化研究[J]. 安徽中医药大学学报, 2022, 41(6): 76-82.
|
|
[10]
|
张伟, 孙叶芬, 金传山, 等. 中药配方颗粒研究现状与展望[J]. 中草药, 2022, 53(22): 7221-7233.
|
|
[11]
|
王菁, 祝浩东. 中药配方颗粒质量控制与发展策略研究[J]. 中医药管理杂志, 2022(17): 216-218.
|
|
[12]
|
魏锋, 程显隆, 荆文光, 等. 中药材及饮片质量标准研究有关问题思考[J]. 中国药学杂志, 2022(18): 1493-1503.
|