公子小丑鱼性逆转过程中性腺的组织学观察

Histological Observation of the Gonads during Amphiprion ocellaris Sex Reversal

摘要: 公子小丑鱼是典型的性逆转物种，其生长发育过程中先是雄性，继而转变为雌性，且性别转化不可逆转。本实验随机选取不同规格(3.8~10.0 cm)的野生公子小丑鱼26条，记录体长和体重，进行石蜡切片和HE染色，将不同时期公子小丑鱼的性腺发育变化与体长和体重耦合起来。分析体长与体重，得到公式y = 1.6943x − 6.2626 (y为体重，x为体长)。研究表明，当公子小丑鱼体长 ≤ 6.9厘米，体重 ≤ 5.4克时，处于雄性阶段，当公子小丑鱼体长 ≥ 6.9厘米，体重 ≥ 5.4克时，处于雌性阶段。本研究对于小丑鱼的亲本构建及育种有一定的参考价值。

Abstract: The Amphiprion ocellaris is a typical sex reversal species, in which the development process begins with males and ends with females, and the sex transition is irreversible. In this experiment, 26 wild Amphiprion ocellaris of different sizes (3.8~10.0 cm) were randomly selected and their body length and weight were recorded, coupling changes in gonadal development at different stages of Amphiprion ocellaris with body length and weight. The formula y = 1.6943x − 6.2626 (y is body weight, x is body length) was obtained by analyzing body length and body weight. Studies have shown that when the Amphiprion ocellaris body length is ≤ 6.9 cm and the body weight is ≤ 5.4 g, they are in the male stage, and when the Amphiprion ocellaris body length is ≥ 6.9 cm and the body weight is ≥ 5.4 g, they are in the female stage. This study has certain reference value for parent construction and breeding of clownfish.