基于网络药理学探究半夏厚朴汤治疗慢性咽炎的作用机制

To Explore the Mechanism of Pinellia Magnolia Decoction in Treating Chronic Pharyngitis Based on Network Pharmacology

摘要: 目的：基于网络药理学方法探究半夏厚朴汤治疗慢性咽炎的作用机制。方法：通过TCMSP (中药系统药理学技术平台)数据库获取“半夏”、“厚朴”、“紫苏”、“茯苓”和“生姜”的主要化学成分，根据ADME筛选出其活性成分及作用靶点，并上传Cytoscape 3.7.2软件构建“药物–成分–靶点网络”。同时通过Gencards、OMIM和DRUGBANK数据库获取慢性咽炎的主要靶点，用在线数据分析作图网站制作Venn图，并将所得交集靶点导入Cytoscape 3.8.2构建“药物–有效成分–靶点–疾病”网络。然后运用STRING数据库绘制蛋白互作(PPI)网络，结果导入Cytoscape软件3.8.2进行网络拓扑分析。最后利用Matescape数据库对交集靶点进行GO功能、KEGG通路富集分析，采用Cytoscape3.8.2构建“靶点–通路”网络。结果：筛选出半夏厚朴汤有效化学成分44个，对应靶点157个，慢性咽炎靶点1288个，半夏厚朴汤慢性咽炎交集靶点85个。分析得半夏厚朴汤治疗慢性咽炎的重要活性成分主要是木犀草素、β-谷甾醇、豆甾醇、黄芩、宽松、卡维定、桉油醇、黄芩苷、塞雷维甾醇，TP53、AKT1、JUN、CASP3、TNF、EGFR、IL6等。GO功能富集分析得到2479项结果，其中BP 2249项，MF 129项、CC 101项。KEGG通路分析得到177条信号通路。结论：半夏厚朴汤治疗慢性咽炎的多成分、多靶点、多通路的作用机制，为治疗慢性咽炎提供了网络药理学依据。

Abstract: Objective: Based on the method of network pharmacology to explore the mechanism of Banxia Houpu decoction in the treatment of chronic pharyngitis. Methods: The main chemical components of Pinellia pinellia, Magnolia officinalis, Perilla, Poria cocos and ginger were obtained from TCMSP database, and the active components and target sites were selected based on ADME, and the drug-composition-target network was constructed by uploaded Cytoscape 3.7.2 software. Meanwhile, major targets of chronic pharyngitis were obtained from Gencards, OMIM and DRUGBANK databases, and Venn diagram was prepared using online data analysis and mapping website. The obtained intersection targets were imported into Cytoscape 3.8.2 to build a “drug-activity-target-disease” network. Then the STRING database is used to draw the PPI network. The results were imported into Cytoscape software 3.8.2 for network topology analysis. Finally, GO function and KEGG pathway enrichment analysis were conducted on the intersection target using Matescape database, and Cytoscape3.8.2 was used to construct the “target-pathway” network. Results: 44 effective chemical components of Pinxia Houpu decoction were screened, 157 corresponding targets, 1288 chronic pharyngitis targets, 85 chronic pharyngitis intersection targets of Pinxia Houpu decoction. Analysis showed that the main active ingredients of Pinellia Houpu decoction for chronic pharyngitis were luteolin, β-sitosterol, stigmasterol, baicalin, carvetin, cineolitol, baicalin, serevisterol, TP53, AKT1, JUN, CASP3, TNF, EGFR, IL6, etc. GO functional enrichment analysis obtained 2479 results, including BP 2249, MF 129 and CC 101. KEGG pathway analysis showed 177 signaling pathways. Conclusion: The multi-component, multi-target and multi-pathway mechanism of Pinellia Magnolia decoction in the treatment of chronic pharyngitis provides the network pharmacological basis for Pinellia Magnolia decoction in the treatment of chronic pharyngitis.