标题:
磷酸盐对盐生杜氏藻生长和细胞色素积累的影响Effects of Phosphate on the Growth and Cell Pigment Accumulation of Dunaliella salina
作者:
刘笑天, 王培磊
关键字:
KH2PO4/sub>, 盐生杜氏藻, 生长, β-胡萝卜素, 吸收规律KH2PO4/sub>, Dunaliella salina, Growth, β-Carotene, Absorpted Patterns
期刊名称:
《Advances in Marine Sciences》, Vol.3 No.3, 2016-09-28
摘要:
研究了不同浓度(0~0.30 mmol/L)的KH2PO4对盐生杜氏藻生长和细胞色素积累的影响，结果表明，添加0.10 mmol/L KH2PO4，细胞生长最快，最高密度为153 × 104 cell/mL，无磷对照组密度最低，仅为36 × 104 cell/mL；KH2PO4浓度为0.20 mmol/L时，β-胡萝卜素积累量最高，为12.8 mg/L，其次是0.15 mmol/L，对照组很低，仅为6.28 mg/L，KH2PO4超过0.25 mmol/L对β-胡萝卜素积累有抑制作用；适当添加KH2PO4对D. salina叶绿素a合成有促进作用；在培养的前6天，pH值明显上升，随后4~5天又下降；添加0.1 mmol/L KH2PO4细胞蛋白质含量最高，为37.34%，对照组仅为24.86%；0.10 mmol/L组的KH2PO4在6天内吸收完毕，KH2PO4吸收的时间与其最初的浓度呈正相关关系，建立了藻液中KH2PO4被盐生杜氏藻吸收的动力学方程。
The effects of KH2PO4 with different levels on the growth and cell pigment accumulation of Dunaliel-la salina were investigated and the results indicated that the maximum growth of 153 × 104 cell/mL was detected in culture medium with 0.1 mmol/L KH2PO4 and the minimum of 36 × 104 cell/mL in the control group. The culture medium with 0.20 mmol/L KH2PO4 had the highest β-carotene con-tent of 12.8 mg/L, followed by 11.04 mg/L with 0.15 mmol/L KH2PO4, and the control group had lower β-carotene content of only 6.28 mg/L. Over 0.25 mmol/L KH2PO4 was found to have depress-ing function to cell growth. Right level KH2PO4 was helpful to chlorophyll a biosynthesis. During the first 6 days within culture period, pH values of the culture media rose up, and then declined in the following 4 - 5 days. The cells which were cultured in media with 0.10 mmol/L KH2PO4 had the high-est protein content of 37.34%, while the control group only had 24.86%; 0.10 mmol/L KH2PO4 was assimilated completely within 6 days, and the uptake time had right-relationship with KH2PO4 con-tents. Finally, the assimilated mechanic equation of KH2PO4 by D. salina was set up.