标题:
植物内生真菌HCCB06030的菌种鉴定和次级代谢产物研究Studies on the Taxonomic Identification and Secondary Metabolites from Endophytic Fungus HCCB06030
作者:
刘崇玉, 杨志钧, 殷瑜, 戈梅, 钱秀萍
关键字:
植物内生菌, 链格孢属, 次级代谢产物Endophytic Fungus; Alternaria sp.; Secondary Metabolites
期刊名称:
《Bioprocess》, Vol.2 No.3, 2012-09-28
摘要:
植物内生真菌的次级代谢产物是生物活性化合物的重要来源之一。本论文对具有肿瘤细胞抑制活性的植物内生真菌菌株HCCB06030进行了菌落和孢子形态特征鉴定，以及ITS序列的相似性分析，确定该菌株为链格孢属真菌。采用乙酸乙酯萃取、硅胶柱层析和高压液相制备等方法对该菌株的代谢产物进行分离纯化，利用紫外、质谱和核磁共振等波谱数据对化合物进行结构解析，确定获得的6个化合物为格链孢酚、dl-aloesol、Solaniol、Isoaltenuene、Altenuene和5-甲氧基格链孢酚。化合物dl-aloesol在链格孢属真菌中首次报道。
The secondary metabolites from endophyte are one of the most important sources for bioactive compounds. In this paper the endophyte strain of HCCB06030, which had the inhibitory activity against tumor cells identified as Alternaria sp. by observing the characteristics of colony and spore and comparing with the sequence of internal transcribed spacer (ITS). The metabolites from the strain of HCCB06030 were isolated by using ethyl acetate extraction, silica gel column chromatography and HPLC preparation, and the chemical structures of the compounds were identified with those of alternariol, dl-aloesol, solaniol, isoaltenuene, altenuene and 5-methoxyl-alternariol by the analyses of their UV, MS and NMR spectra data. The compound of dl-aloesol was first reported in the genus of Alternaria.