标题:
大白菜AP2/EREBP转录因子基因BrABR1的启动子区生物信息学分析Bioinformafics Analysis of Promoter Region of an AP2/EREBP Transcription Factor Gene BrABR1 in Chinese Cabbage (Brassica rapa subsp. Pekinensis)
作者:
吴朝锋, 刘超, 郑慧, 高翔, 邓朝伟, 庹汉兵, 邓川, 邓聿杉, 向浏欣
关键字:
大白菜, BrABR1基因, 启动子, 生物信息学分析Chinese Cabbage, BrABR1 Gene, Promoter, Bioinformatics Analysis
期刊名称:
《Botanical Research》, Vol.5 No.6, 2016-11-28
摘要:
大白菜BrABR1基因属于AP2/EREBP家族转录因子，与植物激素脱落酸(ABA)和胁迫反应相关。BrABR1基因cDNA全长1299 bp，基因登录号为XM_009113908。通过在NCBI上查找获取BrABR1基因的启动子区序列3377 bp片段。利用多种在线相关软件对BrABR1基因的启动子区序列预测知，启动子区存在多个可能的TATA-box和CAAT-box，以及126个顺式作用元件如G-box、I-box等光响应元件、赤霉素响应元件及其他调控元件；含3个可能的核心启动子序列；不含CpG岛。
BrABR1 gene of Chinese cabbage belongs to the AP2/EREBP transcript factor family genes, which is involved in the phytohormone ABA and the stress response. By searching BrABR1 gene in the NCBI, its cDNA sequence was 1299 bp (Gene ID: XM_009113908) and we obtained its 3377 bp promoter region. The results of bioinformatics analysis of the promoter region sequence of the BrABR1 gene by using various SaaS showed that there were several TATA-box and CAAT-box in the promoter region; 162 cis-elements such as G-box, I-box, gibberellin response elements and other regulatory elements were found; there were three possible core promoter sequences, but no CpG island.