标题:
影响火鹤愈伤组织诱导、增殖和芽分化的因素研究Study on Factors Influencing Callus Induction, Proliferation and Bud Differentiation of Anthurium scherzerinum
作者:
杜宝贵, 梁彩红, 黎扬辉, 易懋升, 张志胜
关键字:
火鹤, 愈伤组织诱导, 增殖, 芽分化Anthurium scherzerinum; Callus Induction; Proliferation; Bud Differentiation
期刊名称:
《Hans Journal of Agricultural Sciences》, Vol.2 No.3, 2012-09-27
摘要:
本研究旨在通过研究影响火鹤组培快繁的因素，以其为大规模种苗生产提供参考。试验以Graffity等3个火鹤品种的组培苗为材料，研究了影响火鹤愈伤组织诱导、增殖和芽分化的因素。研究结果表明，3个品种的组培苗叶片愈伤组织诱导率差异不明显；6-BA在无菌苗叶片愈伤组织诱导和分化过程中，是主要影响因素；单独使用2,4-D的愈伤诱导率低，仅为20.84%。在愈伤组织块分化过程中，6-BA + NAA组合的分化率比6-BA + 2,4-D组合的高；不同外植体的愈伤组织诱导率和褐变率差异不显著，叶柄的愈伤组织诱导率最高，为97.22%，叶片次之，根尖的愈伤组织诱导率最低，为93.06%。该试验初步获得了火鹤组培快繁的相关技术体系，为火鹤规模化生产提供了依据。
In order to provide reference to the mass seedling production, the factors influencing the callus induction, proliferation and bud differentiation of Anthurium scherzerinum were studied by using the steriled seedling of 3 varieties such as Graffity as materials. Result: The results showed that the callus inducing rates of the 3 varieties were not obvious. 6-BA is the main influencing factor in the process of callus induction and bud differentiation. The inducing rate by using 2,4-D only was low, 20.84%. In the process of bud dif- ferentiation, the differentiation of combination of 6-BA + NAA is higher than that of combination of 6-BA + 2,4-D. The inducing rate and browning rate of callus were not obvious between different explants, in which the inducing rate of petiole was the highest, 97.22%, followed by leaf, the induction rate of root tip was the lowest, 93.06%. The related technical system of tissue culture of Anthurium scherzerinum was obtained from the test, which providing a basis to the large-scale production.