标题:
基于ALA-PDT的HL60细胞中活性氧的检测ROS Detection in HL60 Based on ALA-PDT
作者:
李莉莉, 郑泽鳞, 黄康强, 熊建文, 王健
关键字:
活性氧, 氨基乙酰丙酸(ALA), 光动力疗法(PDT), 荧光探针Reactive Oxygen Species (ROS); 5-Aminolevulinic Acid (ALA); Photodynamic Therapy (PDT); Fluorescent Probe
期刊名称:
《Optoelectronics》, Vol.1 No.2, 2011-12-28
摘要:
随着光动力学疗法(photodynamic therapy, PDT)基础研究的不断深入和临床应用的广泛开展，对PDT过程中产生的活性氧的实时监测近年来已成为PDT研究的热点问题。实验采用荧光探针技术，通过获得的荧光光谱研究了基于ALA-PDT的HL60细胞中活性氧产生的情况。实验结果表明在ALA浓度为10 mM/L，孵育时间为4 h，光照剂量为60 min的光学参数下，光动力反应中产生的活性氧是最多，HL60细胞灭活效果最佳，PDT效率达93.2%。
With the development of the basic research of photodynamic therapy (PDT) and a wide range of potential clinical applications have be carried out. The monitoring of reactive oxygen species (ROS) which produced in PDT has become a hot issue of PDT research in recent years. In the paper, the status of ROS in HL60 cells were investigated by fluorescence spectra acquired during PDT. The results showed that the largest number of ROS in PDT was achieved at the optimized conditions(the cells incubation time is 4 hours, the added concentration of ALA is 10 mM/L), in which up to 93.2% PDT efficiency for the HL60 Cells can be obtained within 60 minutes.