标题:
SRAP分子技术在植物基因组学研究中的应用
Application of SRAP Molecular Techniques in Plant Genome Research
作者:
李彦锦, 唐婷婷, 刘本文, 江磊, 张磊, 李刚, 覃瑞, 刘虹
关键字:
SRAP, 基因组学, SRAP优化
SRAP; Genomics; SRAP Optimization
期刊名称:
《Botanical Research》, Vol.2 No.3, 2013-07-11
摘要:
SRAP分子标记技术即相关序列扩增多态性分子标记技术，是近年发展起来的一种新型分子标记技术，具有操作简便、共显性高、重复性好、费用低等优点，该技术主要是对基因的编码区进行扩增，定向分析基因的功能序列。SRAP以其独特的引物设计在基因组学上广泛应用于遗传分析、分子遗传图谱的构建以及基因定位与克隆等方面。本文对SRAP的原理特点及其在植物基因组学上的应用进展进行概述。SRAP is a new type of molecular marker technique developed in recent years with which abbreviated form of a name of sequence-related amplified polymorphism. SRAP has the advantages of easy to operate, low cost, high codominant and high repeatability. The main task of SRAP is to amplify the coding region of gene, and analyze the functional sequence of the gene directionally. It was widely used in genetic analysis, the construction of molecular genetic map, gene mapping and genetic clone because of its unique primer design on genomics. Based on the characteristics of SRAP and its applications in plant genomics, we introducted the principles of SRAP briefly in this paper.