标题:
不同产地和不同生长期白芍质量研究Study on Quality of Radix Paeoniae Alba of Different Origin and Different Growth Stages
作者:
李越峰, 严兴科
关键字:
白芍, 芍药苷, 薄层色谱, 高效液相色谱, 质量Radix Paeoniae Alba; Paeontflortn; TLC; HPLC; Quality
期刊名称:
《Traditional Chinese Medicine》, Vol.1 No.3, 2012-08-30
摘要:
采用HPLC法测定不同白芍药材中芍药苷含量，确定哪种白芍含芍药苷最高。方法：采用色谱柱：Hypersil C18柱(4.6 × 200 mm，5 μm)；流动相：乙腈/0.05%磷酸水(14:86)；检测：UV 230 nm；流速：1.0 ml/min；柱温：25℃。结果表明：2年生、10月份采收贮存期短的亳白芍芍药苷含量最高。该方法简便，准确可靠，重复性好。
To compare the quality on different Radix Paeoniae Alba using HPLC methods. Column: Hypersil C18 column (4.6 × 200 mm, 5 μm); mobile phase: acetonitrile-H2O (acidified to 0.05% with phosphoric acid) (14:86); UV test: 230 nm; flow-rate: 1.0 ml/min; column temperature: 25˚C. There was the highest content of paeoniflorin in Radix Paeoniae Alba of two-year-growing time, harvest time in October and short storage time from Bozhou. The assay methods are simple, rapid and with good reproducibility.