Upfoff, H.U., Felske, A., Fehr, W. and Wagner-Döbler, I. (2001) The microbial diversity in picoplankton enrichment cultures: A molecular screening of marine isolates. FEMS Microbiology Ecology, 35, 249-258.
未培养微生物的限制因素及培养方法研究进展Progress on the Research Pertinent to Cultivation Constraint Factors and Improved Cultivation Methods of Environmental Uncultured Microorganisms
牛丽纯, 孙玉芳, 赵天琦, 宋福强
未培养微生物, 限制因素, 培养方法Uncultured Microorganisms, Limiting Factors, Culture Method
《Advances in Microbiology》, Vol.3 No.2, 2014-06-12
A great deal of unique uncultured microorganisms exist in various kinds of habitats; they benefit humans but are difficult to be purely cultured and are rich in species diversity, playing a very important role on the matter cycle of the whole earth and life sustainability. The uncultured microorganisms as well as the factors attributing to their currently uncultured feature were briefly introduced in this review, and subsequently novel methods and improved measurements for isolating them were highlighted. Currently, novel medium containing microorganism recovery promoting factors was designed through simulating maintaining the relationships among microbes under natural conditions. Combination of a variety of proven technologies is employed to make the pure culture possible of uncultured microorganisms, which play a promoting role on the development of microbiological field.