标题:
多西紫杉醇协同卡铂对骨肉瘤细胞MG-63细胞毒性的实验研究Research the Cytotoxicity of Docetaxel Combined with Carboplatin in Osteosarcoma Cell Line MG-63
作者:
谭泽恩, 董乐乐
关键字:
骨肉瘤细胞, 多西紫杉醇, 卡铂, 细胞毒性作用Osteosarcoma, Docetaxel, Carboplatin, Sytotoxicity
期刊名称:
《Advances in Clinical Medicine》, Vol.5 No.1, 2015-03-30
摘要:
目的：研究多西紫杉醇和卡铂联用对骨肉瘤细胞MG-63的增殖抑制作用，寻找新的骨肉瘤临床治疗方案。方法：采用MTT法细胞毒性试验，测定多西紫杉醇及卡铂对MG-63细胞株的抑制率并计算其IC50值，根据所测得的IC50值，分别用IC50多西紫杉醇、IC50卡铂、1/2 IC50多西紫杉醇 + 1/2 IC50卡铂作用于MG-63细胞株24 h、48 h、72 h，比较三组药物处理方法对MG-63细胞株的抑制率。结果：根据相应实验结果制作生长曲线通过公式IC50 = lg − 1[Xm − i(ΣP − 0.5)]求得两药的IC50值分别为0.25 mg/ml和4.00 mg/ml。分别用0.25 mg/ml多西紫杉醇、4 mg/ml卡铂、0.125 mg/ml多西紫杉醇+2 mg/ml卡铂与骨肉瘤细胞株MG-63作用24 h时，其细胞毒性指数(CI)分别为：38.6%、48.7%和60.0%，3组比较差异有统计学意义(P 0.05)，联用组与另外两组比较差异均由统计学意义(P Objective: To research the cytotoxicity of docetaxel combined with carboplatin on osteosarcoma cell line MG-63. Methods: Using MTT assay, we obtained the mean IC50 of docetaxel and caboplatin for the osteosarcoma cell line MG-63. The MG-63 was incubated with IC50 docetaxel, IC50 carboplatin and 1/2 IC50 docetaxel + 1/2 IC50 carboplatin for 24 h, 48 h, 72 h. The cytotoxicity of each group was measured by MTT assays. Result: By the formula IC50 = lg − 1[Xm − i(ΣP − 0.5)] to obtain the IC50 of docetaxel and carboplatin for osteosarcoma cell MG-63 were 0.25 mg/ml and 4.00 mg/ml respectively. The mean cytotoxicity index (CI) of 0.25 mg/ml docetaxel, 2 mg/ml carboplatin and 0.125 mg/ml docetaxel + 2 mg/ml carboplatin for 24 h were 38.6%, 48.7% and 60.0% respectively (P 0.05; the combined group compared with the other two groups P