AAC  >> Vol. 3 No. 2 (May 2013)

    Research Progress in Development of the Determination Methods of Vitamine C in Food and Pharmaceuticals

  • 全文下载: PDF(276KB) HTML   XML   PP.9-14   DOI: 10.12677/AAC.2013.32003  
  • 下载量: 2,220  浏览量: 11,544   科研立项经费支持



维生素C食品与药品检测方法综述Vitamin C; Food and Pharmaceuticals; Determination Method; Review



Vitamin C is necessary to the growth and metabolism for human beings. Vitamin C is mainly from fruits, vegetables and medicine, so it is one of the important standards to evaluate the quality of the food nutrition and medicine. With the growing concern of food safety, the quantitative analysis of vitamin C in food nutrition and pharmaceuticals has attracted more and more attention. In recent years, determination techniques of vitamin C in food and pharma- ceuticals have been developed rapidly, including titrimetric method, spectrophotometric method, electrochemical me- thod, chemiluminescence method, flow injection analysis, liquid chromatography and indirect atomic adsorption me- thods, etc. In this work, some principles, characteristic and the recent progress of reported methods have been summarized and compared.


朱宏亮, 戴火锋, 汪凯闰, 姚屠鹏, 张蕴, 沈昊宇. 食品与药品中维生素C含量的检测方法进展[J]. 分析化学进展, 2013, 3(2): 9-14. http://dx.doi.org/10.12677/AAC.2013.32003


[1] M. W. Davey, M. V. Montagu, D. Inze, et al. Review plant L-ascorbic acid: Chemistry, function, metabolism, bioavailabil- ity and effects of processing. Journal of the Science of Food and Agriculture, 2000, 80: 825-860.
[2] J. S. Hampl, C. A. Taylor and C. S. Johnston. Intakes of vitamin C, vegetables and fruits: Which schoolchildren are at risk. Jour- nal of the American College of Nutrition, 1999, 18(6): 582-590.
[3] 中国营养学会. 中国居民膳食营养素参考摄入量[M]. 北京: 中国轻工业出版社, 2000, 445-450.
[4] L. Novakova, P. Solich and D. Solichova. HPLC methods for simultaneous determination of ascorbic and dehydroascorbic acids. Trends in Analytical Chemistry, 2008, 27: 942-958.
[5] S. K. Lee, A. A. Kader. Preharvest and postharvest factors influencing vitamin C content of horticultural crops. Postharvest Biology and Technology, 2000, 20: 207-220.
[6] S. P. Arya, M. Mahajan, P. Jain. Non-spectrophotometric methods for the determination of Vitamin C. Analytica Chimica Acta, 2000, 417: 1-14.
[7] 吴春艳. 水果中维生素C含量的测定及比较[J]. 武汉理工大学学报, 2007, 29: 90-91.
[8] 王元秀, 庄海燕. 微量滴定法测定猕猴桃中维生素C的含量[J]. 济南大学学报, 2001, 15: 374-378.
[9] 陈焕文, 宋庆宇, 刘宏伟等. 测定抗坏血酸的亚甲蓝褪色光度法[J]. 分析测试学报, 2001, 20(2): 55-57.
[10] M. A. Farajzadeh. S. J. Nagizadeh. A simple and reliable spec-trophotometric method for the determination of ascorbic acid in pharmaceutical preparations. Analytical Chemistry, 2003, 8(10): 927-932.
[11] E. K.Janghel. V. K. Gupta. M. K.Rai, et al. Micro determination of ascorbic acid using methyl viologen. Talanta, 2007, 72: 1013- 1016.
[12] 黄仲鑫. 袁明松. 王梅华. 果蔬、饮料中抗坏血酸的新亚铜灵光度分析法[J]. 分析测试学报, 1993, 12(4): 63-65.
[13] M. J. Esteve. R. Farre. A. Frigola, et al. Comparison of voltammetric and high performance liquid chromatographic methods for ascorbic acid determination in infant formulas. Food Chemistry, 1995, 52: 99-102.
[14] M. G. Hosseini, M. Faraji and M. M. Momeni. Application of titanium oxide nanotube films containing gold nanoparticles for the electroanalytical determination of ascorbic acid. Thin Solid Films, 2011, 519: 3457-3461.
[15] T. Kato, O. Ohno, T. Nagoshi, et al. Determination of small amounts of L-ascorbic acid using the chemiluminescence of an iron-chlorophyllin complex. Analytical Sciences, 2005, 21: 579- 581.
[16] Y. P. Wen, X. M. Duan, J. K. Xu, et al. One-step electrosynthesis of poly(3,4-ethylenedioxy-thiophene)-ethylsulfate matrix for fa- bricating vitamin C electrochemical biosensor and its determina- tion in commercial juices. Chinese Journal of Polymer Science, 2012, 30(3): 460-469.
[17] L. Ozcan, M. Sahin and Y. Sahin. Electrochemical preparation of a molecularly imprinted polypyrrole-modified pencil graphite electrode for determination of ascorbic acid. Sensors, 2008, 8: 5792-5805.
[18] B. Rezaei, A. A. Ensafi and S. Nouroozi. Flow-injection determination of ascorbic acid and cysteine simultaneously with spectrofluorometric detection. Analytical Sciences, 2005, 21: 1067-1071.
[19] M. C. Yebra-Biurrun. Flow injection determina-tion methods of ascorbic acid. Talanta, 2000, 52: 367-383.
[20] H. J. Iwase. Use of nucleic acids in the mobile phase for the determination of ascorbic acid in foods by high-performance liquid chromatography with electrochemical detection. Journal of Chromatography A, 2000, 881: 327-330.
[21] A. M. Maia, A. R. Baby, W. J. Yasaka, et al. Validation of HPLC stability-indicating method for vitamin C in semisolid pharmaceutical/cosmetic preparations with glutathione and sodium metabisulfite, as antioxidants. Talanta, 2007, 71:639-643.
[22] A. Tai, E. J. Gohda. Determination of ascorbic acid and its re- lated compounds in foods and beverages by hydrophilic interact- tion liquid chromatography. Journal of Chromatography B, 2007, 853: 214-220.
[23] A. R. B. de Quiros, M. Fernandez-Arias and J. Lopez-Hernandez. A screening method for the determination of ascorbic acid in fruit juices and soft drinks. Food Chemistry, 2009, 116: 509-512.
[24] S. Nojavan, K. F. M. Kiaie, et al. Extraction and quantitative determination of ascorbic acid during different maturity stages of Rosa canina L. fruit. Journal of Food Composition and Ana- lysis, 2008, 21: 300-305.
[25] V. Spinola, B. Mendes, J. S. Camara, et al. An improved and fast UHPLC-PDA methodology for determination of L-ascorbic and dehydroascorbic acids in fruits and vegetables. Evaluation of degradation rate during storage. Analytical and Bioanalytical Chemistry, 2012, 403: 1049-1058.
[26] P. Emadi-Konjin, Z. Verjee, A. V. Levin, et al. Measurement of intracellular vitamin C levels in human lymphocytes by reverse phase high performance liquid chromatography (HPLC). Clinical Biochemistry, 2005, 38: 450-456.
[27] 曹志广, 丁祥欢, 叶建农. 维生素片中水溶性维生素的毛细管电泳–电化法测定[J]. 分析测试学报, 1999, 18(5): 57-59.
[28] 刘国胜, 张玉铭, 王珏云等. 用原子吸收间接法测定水果蔬菜和果汁饮料中的维生素C[J]. 河北大学学报, 1999, 19(3): 260-263.
[29] M. Noroozifar, M. Khorsani-Motlagh and K. Akhavan. Atomic absorption spectrometry for the automatic indirect determination of ascorbic acid based on the reduction of manganese dioxide. Analytical Sciences, 2005, 21: 655-659.
[30] Y. C. Jiang, Z. Q. Zhang and J. Zhang. Flow-injection, on-line concentrating and flame atomic absorption spectrometry for indirect determination of ascorbic acid based on the reduction of iron(III). Analytica Chimica Acta, 2001, 435: 351-355.
[31] 杨建洲, 张荣莉. 毛细管区带电泳分析果蔬中的维生素C含量[J]. 西北轻工业学院学报, 2001, 19(3): 12-14.
[32] GB/T 5009.159-2003. 食品中还原型抗坏血酸的测定[S]. 中华人民共和国卫生部, 2003.